NCRR: UCSC Acquisition of a Thermo Electron LTQ-Mass Spectrometer

NCRR：UCSC 采购热电子 LTQ 质谱仪

• 批准号: 7046277
• 负责人: Theodore R Holman
• 金额: $ 36.85万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA CRUZ
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7046277
• 关键词: biochemistry; biotechnology; environmental toxicology; housing; ions; mass spectrometry; performance; play; protein structure function; proteomics; role; toxicology; university

DESCRIPTION (provided by applicant): Thirteen UCSC faculty members have come together to develop this application for the purchase of a state-of-the-art Finnigan LTQ Mass Spectrometer System (LTQ MS). This system consists of a high- performance 2D linear quadrupole ion trap mass spectrometer (LTQ). We are asking the NCRR to fund the purchase of this system to greatly expand and modernize our Mass Spectrometry MS Facility, which is dedicated to research in UCSC's Departments of Chemistry and Biochemistry (CBC), Molecular, Cell, and Developmental Biology (MCD), and Environmental Toxicology (ETOX). The shared MS Facility currently houses two mass spectrometers, while 2 additional mass spectrometers are housed in individual labs and available on request to the biomedical research faculty. While these instruments are well maintained, each has limitations that undercut its ability to support the quality and breadth of research that could otherwise be undertaken at this university. Without additional, updated equipment, our ability to provide our faculty with a first class research infrastructure will remain compromised. This problem will only become exacerbated in the future, as the biomedical research faculty of these four departments is projected to expand by 50% over the next decade. Acquisition of the LTQ MS will play a large role in alleviating these problems. The research initiatives of the ten major users can be divided into two broad areas. Each has far reaching implications for basic biological science, as well as for related biotechnology applications and the treatment of disease. The broad subject areas include: 1) ESI-Linear Ion-Trap based proteomics to study complex biochemical processes 2) ESI-Linear Ion-Trap approaches to understanding signal transduction pathways. The five major users in Area 1 (Fink, Holman, Lokey, Millhauser, and Smith) use proteomic methods on projects that depend on the resolution and sensitivity of the proposed instrument. Five other major users associated with Area 2 (Hartzog, Kellogg, Noller, Ottemann, and Tamkun) utilize this technology for biochemical cellular machinery characterization. Three Minor Users who will employ the instrument to investigate protein structure and function, and to clarify cellular mechanisms involved toxicology.

描述（由申请人提供）： 13 名 UCSC 教员共同开发了此应用程序，用于购买最先进的 Finnigan LTQ 质谱仪系统 (LTQ MS)。该系统由高性能 2D 线性四极杆离子阱质谱仪 (LTQ) 组成。我们请求 NCRR 资助购买该系统，以极大地扩展和现代化我们的质谱 MS 设施，该设施致力于 UCSC 化学和生物化学 (CBC)、分子、细胞和发育生物学 (MCD) 系的研究，和环境毒理学（ETOX）。共享的 MS 设施目前拥有两台质谱仪，另外 2 台质谱仪位于各个实验室，可根据生物医学研究人员的要求提供。虽然这些工具维护得很好，但每个工具都有局限性，削弱了其支持本大学本来可以进行的研究的质量和广度的能力。如果没有额外的更新设备，我们为教师提供一流研究基础设施的能力仍将受到损害。这个问题在未来只会变得更加严重，因为这四个系的生物医学研究人员预计在未来十年内将扩大 50%。 LTQ MS的购置将为缓解这些问题发挥重要作用。十大用户的研究举措可分为两大领域。每一项都对基础生物科学以及相关生物技术应用和疾病治疗产生深远影响。广泛的学科领域包括：1) 基于 ESI-线性离子阱的蛋白质组学，用于研究复杂的生化过程 2) ESI-线性离子阱方法，用于了解信号转导途径。区域 1 的五个主要用户（Fink、Holman、Lokey、Millhauser 和 Smith）在依赖于拟议仪器的分辨率和灵敏度的项目中使用蛋白质组学方法。与 Area 2 相关的其他五个主要用户（Hartzog、Kellogg、Noller、Ottemann 和 Tamkun）利用该技术进行生化细胞机械表征。三个小用户将使用该仪器研究蛋白质结构和功能，并阐明涉及毒理学的细胞机制。

项目成果

Characterization of an anthraquinone fluor from the bioluminescent, pelagic polychaete Tomopteris.

来自生物发光的远洋多毛动物 Tomopteris 的蒽醌荧光体的表征。

• 发表时间: 2014-12
• 作者: Francis, Warren R;Powers, Meghan L;Haddock, Steven H D
• 通讯作者: Haddock, Steven H D

Error rates for nanopore discrimination among cytosine, methylcytosine, and hydroxymethylcytosine along individual DNA strands.

纳米孔沿单个 DNA 链区分胞嘧啶、甲基胞嘧啶和羟甲基胞嘧啶的错误率。

• 发表时间: 2013-11-19
• 影响因子: 11.1
• 作者: Schreiber, Jacob;Wescoe, Zachary L;Abu;Vivian, John T;Baatar, Baldandorj;Karplus, Kevin;Akeson, Mark
• 通讯作者: Akeson, Mark

Early doors (Edo) mutant mouse reveals the importance of period 2 (PER2) PAS domain structure for circadian pacemaking.

早期门 (Edo) 突变小鼠揭示了周期 2 (PER2) PAS 结构域结构对于昼夜节律起搏的重要性。

• 发表时间: 2016-03-08
• 影响因子: 11.1
• 作者: Militi, Stefania;Maywood, Elizabeth S;Sandate, Colby R;Chesham, Johanna E;Barnard, Alun R;Parsons, Michael J;Vibert, Jennifer L;Joynson, Greg M;Partch, Carrie L;Hastings, Michael H;Nolan, Patrick M
• 通讯作者: Nolan, Patrick M

A Slow Conformational Switch in the BMAL1 Transactivation Domain Modulates Circadian Rhythms.

BMAL1 反式激活域中的缓慢构象开关调节昼夜节律。

• DOI: 10.1016/j.molcel.2017.04.011
• 发表时间: 2017-05-18
• 期刊: Molecular cell
• 影响因子: 16
• 作者: Gustafson CL;Parsley NC;Asimgil H;Lee HW;Ahlbach C;Michael AK;Xu H;Williams OL;Davis TL;Liu AC;Partch CL
• 通讯作者: Partch CL