Functional micro-architecture of the visual cortex

视觉皮层的功能微结构

基本信息

批准号：
7482255
负责人：
R CLAY REID
金额：
$ 59.03万
依托单位：
HARVARD MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-04-01 至 2010-08-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The goal of the proposed studies is to understand the functional organization of layers 2/3 in visual cortex of cats at the level of single neurons. Functional architecture of visual cortex has been studied with two approaches: (1) electrophysiology of single neurons, and (2) optical imaging, which provides functional maps at low resolution: >100 mu/m. The new technique of calcium imaging with two-photon laser-scanning microscopy (resolution <1 mu/m) bridges the gap between single-unit electrophysiology and conventional optical imaging. Using this technique, we will create the first maps of visual cortical function at the scale of single neurons. We call cortical organization at this level functional micro-architecture. We will examine functional micro-architecture with a three-pronged strategy: intrinsic-signal imaging, two-photon imaging, and electrophysiology. We will first obtain maps of visual cortex with conventional optical imaging, then zoom in with two-photon microscopy to examine smaller regions at single-cell resolution. In Aim 1, we will examine the fine-scale clustering of neurons according to receptive-field attributes such as orientation selectivity, direction selectivity, ocular dominance and retinotopy. In Aim 2, we will combine single-cell calcium imaging with electrophysiology: to calibrate the calcium signal, to examine co-active ensembles of neurons and (with intracellular stimulation) to determine the influence of one neuron on its potential targets in the circuit.

描述（由申请人提供）：拟议研究的目的是了解单个神经元水平的猫视觉皮层中层2/3的功能组织。已经研究了视觉皮层的功能结构，采用了两种方法：（1）单神经元的电生理学和（2）光学成像，该光学成像以低分辨率提供功能图：> 100 mu/m。用两光子激光扫描显微镜（分辨率<1 mu/m）钙化成像的新技术弥合了单单元电生理学和常规光学成像之间的间隙。使用此技术，我们将在单个神经元的尺度上创建视觉皮质功能的第一张地图。我们称此级别的皮质组织功能微体系结构。我们将通过三支策略来检查功能性微体系结构：内在信号成像，两光子成像和电生理学。我们将首先获得具有常规光学成像的视觉皮层图，然后用两光子显微镜放大，以单细胞分辨率检查较小的区域。在AIM 1中，我们将根据接收场属性（例如方向选择性，方向选择性，眼优势和视网膜）来检查神经元的细尺度聚类。在AIM 2中，我们将将单细胞钙成像与电生理学相结合：校准钙信号，检查神经元的共同聚合物，并（使用细胞内刺激）确定一个神经元对其电路潜在目标的影响。