Fundamental Processes in Electron Capture Dissociation: Peptides, Polymers and Fullerenes

电子捕获解离的基本过程：肽、聚合物和富勒烯

• 批准号: EP/E00329X/1
• 负责人: Helen Cooper
• 金额: $ 35.53万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FE00329X%2F1
• 关键词: Fundamental; Processes; Electron; Capture; Dissociation

A molecule's structure defines its reactivity and, in the case of biomolecules, its function. It is therefore important to be able to elucidate molecular structure. Mass spectrometry is a technique which enables measurement of the mass of a molecule. Structural information may be gleaned by fragmenting the molecule and measuring the masses of its constituent parts. Dissociation of a molecule followed by mass measurement of the fragments is known as tandem mass spectrometry. Several established techniques exist for inducing fragmentation however each has limitations, such as the inability to cleave certain chemical bonds.Electron capture dissociation (ECD) is a recently developed tandem mass spectrometry technique which shows a number of advantages for the structural analysis of peptides and proteins. However, the mechanism by which ECD occurs is not clearly defined and we cannot be certain that we are realising the potential of this analytical technique. Furthermore, the ECD of other molecular structures has not been comprehensively addressed.The proposed work will address ECD as an analytical technique in a broader context than previous investigations. We will define the ECD behaviour of particular peptides, polymers and fullerenes. The main outcome will be a better understanding of the mechanisms of ECD. As a result, it will be possible to optimise ECD performance and gain maximum structural information for a variety of molecular structures.

分子的结构定义了其反应性，并且在生物分子的情况下是其功能。因此，重要的是能够阐明分子结构。质谱法是一种能够测量分子质量的技术。可以通过碎片分子并测量其组成部分的质量来收集结构信息。分子的解离，然后对片段进行质量测量，称为串联质谱法。有几种既定的技术来诱导碎片化，但是每个技术都有局限性，例如无法裂解某些化学键。电子捕获解离（ECD）是一种最近开发的串联质谱技术，显示了肽和蛋白质结构分析的许多优势。但是，ECD发生的机制尚未明确定义，我们无法确定我们正在意识到这种分析技术的潜力。此外，其他分子结构的ECD尚未得到全面解决。拟议的工作将在更广泛的环境中作为一种分析技术来解决比以前的研究更广泛的分析技术。我们将定义特定肽，聚合物和富勒烯的ECD行为。主要结果将是对ECD机制的更好理解。结果，有可能优化ECD性能并获得各种分子结构的最大结构信息。

项目成果

The radical ion chemistry of S-nitrosylated peptides.

S-亚硝基化肽的自由基离子化学。

• DOI: 10.1007/s13361-012-0492-x
• 发表时间: 2012
• 期刊: Journal of the American Society for Mass Spectrometry
• 影响因子: 3.2
• 作者: Jones AW

Probing the mechanisms of electron capture dissociation mass spectrometry with nitrated peptides.

• DOI: 10.1039/c0cp00623h
• 发表时间: 2010-11-07
• 期刊: Physical chemistry chemical physics : PCCP
• 作者: Jones AW;Cooper HJ
• 通讯作者: Cooper HJ