High performance mass spectrometry at the University of Birmingham

伯明翰大学的高性能质谱仪

• 批准号: BB/M012492/1
• 负责人: Helen Cooper
• 金额: $ 54.18万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM012492%2F1
• 关键词: performance; mass; spectrometry; University; Birmingham

Mass spectrometry is well-established as a technique for characterisation of molecular structure. The chief reason for the success of mass spectrometry is that every molecule has a mass, unlike other properties sometimes measured in bioanalytical chemistry, such as a magnetic moment or absorbance of light at a particular wavelength. The mass of a molecule depends on the composition of its atoms, as each atom has a unique mass. As long as the accuracy of the mass spectrometer is high enough, then measurement of a molecule's mass allows determination of the elemental composition. The more complex the sample, either in terms of the number of components or in terms of the size of the molecule (the number of atoms), the more challenging is mass analysis. This fact is due to overlapping signals from different molecules or versions of the same molecule (isotopomers) and in order to address the problem, a very high resolution mass spectrometer is required.Biological research is overflowing with sample types that require high resolution mass spectrometry: Metabolomic samples contain thousands of low molecular weight species. Proteins are large molecules associated with a vast array of chemical modifications, and which form loosely-bound complexes with themselves, other proteins and other molecule types. The aim of this proposal is to make available the latest developments in the highest performance type of mass spectrometry - Fourier transform ion cyclotron resonance mass spectrometry - to biological research at the University of Birmingham and beyond. The proposal brings together a broad range of researchers from diverse disciplines to address several key strategic priorities for BBSRC (Healthy ageing across the life course, Synthetic biology, Combatting antimicrobial resistance, Food, nutrition & health, Technology for the biosciences).

质谱学是一种表征分子结构的技术。质谱成功的主要原因是每个分子具有质量，与有时在生物分析化学中测量的其他特性不同，例如磁矩或在特定波长处的光吸光度。分子的质量取决于其原子的成分，因为每个原子都有独特的质量。只要质谱仪的准确性足够高，分子质量的测量就可以测定元素组成。样本越复杂，就成分的数量或分子的大小而言（原子数）而言，质量分析越具有挑战性。这一事实是由于来自不同分子或同一分子（同位素异位体）的重叠信号，并且为了解决该问题，需要一个非常高分辨率的质谱仪。生物学研究溢出了需要高分辨率质谱的样本类型：代谢样品含有成千上万的低分子量。蛋白质是与大量化学修饰相关的大分子，它们与自身，其他蛋白质和其他分子类型形成了松散结合的复合物。该建议的目的是使伯明翰大学及其他地区的生物学研究以最高性能类型的质谱质量测量最高类型的最新发展 - 傅立叶转化离子回旋共振质谱法。该提案汇集了各种各样的研究人员，从不同的学科介绍了BBSRC的几个关键战略重点（整​​个生命过程中的健康衰老，合成生物学，打击抗菌素耐药性，食品，营养与健康，生物镜家的技术）。

项目成果

Structural Analysis of 14-3-3-ζ-Derived Phosphopeptides Using Electron Capture Dissociation Mass Spectrometry, Traveling Wave Ion Mobility Spectrometry, and Molecular Modeling.

使用电子捕获解离质谱、行波离子淌度光谱和分子建模对 14-3-3-γ-衍生磷酸肽进行结构分析。

• DOI: 10.1021/acs.jpcb.9b08506
• 发表时间: 2020
• 期刊: The journal of physical chemistry. B
• 作者: Simmonds,AnnaL;Lopez-Clavijo,AndreaF;Winn,PeterJ;Russell,DavidH;Styles,IainB;Cooper,HelenJ
• 通讯作者: Cooper,HelenJ

Workflow for fast lipid tissue screening using LESA-FT-ICR-MS

• DOI: 10.1039/c8ay02739k
• 发表时间: 2019-05-14
• 期刊: ANALYTICAL METHODS
• 影响因子: 3.1
• 作者: Haler, Jean R. N.;Sisley, Emma K.;Fernandez-Lima, Francisco
• 通讯作者: Fernandez-Lima, Francisco

Electron capture dissociation mass spectrometry of phosphopeptides: Arginine and phosphoserine

• DOI: 10.1016/j.ijms.2015.07.024
• 发表时间: 2015-11-15
• 期刊: INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
• 影响因子: 1.8
• 作者: Lopez-Clavijo, Andrea F.;Duque-Daza, Carlos A.;Cooper, Helen J.
• 通讯作者: Cooper, Helen J.