Mouse Core

鼠标核心

• 批准号: 7336594
• 负责人: Edward K. Wakeland
• 金额: $ 26.76万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7336594
• 关键词: Biomedical Research; Breeding; Censuses; Collection; Communities; Congenic Strain; Daily; Gene Targeting; Genotype; Human Resources; Inbred Strain; Individual; Maintenance; Modeling; Mouse Strains; Mus; Records; Research Personnel; Shipping; Ships; Transgenes; Transgenic Organisms; cohort; congenic; novel; programs; research study

The mouse core will be responsible for breeding and providing all mice needed by the Projects within the Program project. The anticipated usage of this core by these three Projects will be: Project 1 (40%), Project 2 (30%), Project 3 (30%). This mouse core will be a component of the Wakeland mouse colony (WMC), which is an SPF colony containing >1500 cages (~5000 daily census). This core has two specific aims. The first specific aim will be to maintain all of the various mouse strains required by PPG investigators and to produce and provide mice for use in all experiments. This will require the maintenance of more than 40 unique strains of mice. The core will be responsible for maintaining breeding records, validating the genotypes of all the B6-congenic strains, and producing experimental cohorts of mice as needed for experiments by personnel in Projects 1, 2, and 3. The second specific aim will be to produce and genotype the new strains that will be produced by all of the projects. This will include breeding all transgene founder strains and introgressing the selected transgene founders onto specific genotypes. The core is also responsible for breeding and shipping mice to other investigators in the biomedical research community requesting access to the mice in our collection.

鼠标核心将负责繁殖，并提供所有项目所需的所有小鼠 计划项目。这三个项目对此核心的预期用法将是：项目1（40％），项目 2（30％），项目3（30％）。该鼠标核心将是Wakeland小鼠菌落（WMC）的组成部分， 这是一个含有> 1500个笼子的SPF菌落（每天约5000个人口普查）。该核心有两个具体的目标。这 第一个具体目的是维护PPG研究者要求的所有各种小鼠菌株和 生产并提供用于所有实验的小鼠。这将需要维护40多个 独特的小鼠菌株。核心将负责维护育种记录，验证 所有B6-综合菌株的基因型，并根据需要产生小鼠的实验人群 项目1、2和3中人员的实验。第二个具体目的是生产和基因型 所有项目都将产生的新菌株。这将包括繁殖所有转基因创始人 菌株和渗入选定的转基因创始人在特定的基因型上。核心也是 负责生物医学研究界的其他调查人员的繁殖和运输老鼠 要求访问我们收藏中的老鼠。