DHA in Pregnancy and Immune Function in Mexican Infants

DHA 在妊娠期和墨西哥婴儿免疫功能中的作用

• 批准号: 6967372
• 负责人: USHA RAMAKRISHNAN
• 金额: $ 6.58万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6967372
• 关键词: DHA; Pregnancy; Immune; Function; Mexican

DESCRIPTION (provided by applicant): We are conducting a randomized, double-blinded controlled trial among pregnant women in Cuernavaca, Mexico, to study the effect of daily supplementation with 200 mg docosahexaenoic acid (DHA) until delivery on a) birth outcomes b) maternal blood and breast milk DMA levels c) postnatal growth and development and d) infant DHA status. This is a collaborative effort among the Department of International Health, Emory University, and the Institute Nacional de Salud Publica in Mexico. The focus of this supplemental study is to evaluate the effect of DHA supplementation on infant immune function. We will analyze infant plasma and maternal breast milk samples currently being collected as part of the parent study protocol. DHA and other n-3 polyunsaturated fatty acids have been shown to modulate immune function in humans; however, few studies have addressed the independent effect of DHA. The central hypothesis of the supplemental study, that maternal DHA supplementation in pregnancy will modulate infant immune function, will be tested by measuring indicators of infant immune function including total immunoglobulin (Ig) concentrations and IgG sub-class concentrations in plasma, to be measured at birth (cord blood) and at 3 months. Antigen-specific antibody response after routine vaccination with 1 dose of diphtheria vaccine will be measured in infants at 3 months. We will measure breast milk IgA concentrations at 1 and 3 months post-partum. The sample size fixed by the parent study will be 116 mother-child pairs per group. The immunological assessments will be done at no cost to the study by the Centers for Disease Control, Atlanta. Data analysis will include group comparisons (intent-to-treat) after ensuring effectiveness of randomization. Adjusted comparisons (using co-variance analysis) will be made if control for potential confounders is required. To our knowledge, there are no studies that have evaluated the impact of maternal DHA supplementation in pregnancy on infant immune function.

描述（由申请人提供）：我们正在墨西哥Cuernavaca对孕妇进行一项随机，双盲对照试验，以研究每天补充200 mg docosahexaenoic（DHA）的效果，直到分娩对a）对a的送达b）b）出生含量b）b）b）母乳和母乳液位C）c）c）c）生长和发育后的生长和dha。这是国际卫生部，埃默里大学和墨西哥的Nacional de Salud Publica的合作努力。这项补充研究的重点是评估补充DHA对婴儿免疫功能的影响。我们将分析婴儿血浆和母乳样品目前正在作为父母研究方案的一部分。 DHA和其他N-3多不饱和脂肪酸已显示可调节人类的免疫功能。但是，很少有研究解决了DHA的独立效应。补充研究的中心假设是，妊娠中补充母体DHA将调节婴儿免疫功能，通过测量婴儿免疫功能的指标，包括总体免疫球蛋白（Ig）浓度（Ig）浓度和IgG亚级浓度，并在出生（绳索血液）和3个月中测量。常规疫苗接种后用1剂白喉疫苗的抗原特异性抗体反应将在婴儿3个月时在婴儿中测量。我们将在产后1和3个月时测量母乳IgA浓度。父母研究固定的样本量将为每组116个母子对。亚特兰大疾病控制中心将无需对研究进行免疫评估。数据分析将包括在确保随机化有效性后的组比较（意图对治疗）。如果需要控制潜在的混杂因素，将进行调整后的比较（使用共同变异分析）。据我们所知，尚无研究评估孕妇补充剂对妊娠对婴儿免疫功能的影响。