A new sensor for bacteria using binding or specific degradation of stimuli responsive polymers

利用刺激响应聚合物的结合或特异性降解的新型细菌传感器

基本信息

批准号：
EP/D065046/1
负责人：
Stephen Rimmer
金额：
$ 79.25万
依托单位：
University of Sheffield
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2007
资助国家：
英国
起止时间：
2007 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=EP%2FD065046%2F1
关键词：
new sensor bacteria using binding

项目摘要

The project will investigate the use of a smart polymer for the detection of bacteria. The underlying principle of this work is that the binding of the chain ends of the hyperbranched polymers can be used to attach the polymer to the cell surface of bacteria. In some cases this binding can progress the polymer through a critical conformational change, in which the polymer changes, in aqueous soloution, from an open solvated coil to a tight non-solvated globule. In other instances the conformational change can be brought about after binding by a small temperature change. Furthermore, careful design of the chain-end ligand can be used to ensure that binding can be formulated to be specific to individual sites on different species of bacteria,. The change from open coil to tight globule can be detected by monitoring fluoresence from chemical labels situated on the polymer chain . These labels report on the chain's conformation by a change in fluoresence. The project will investigate the use of both soluble polymers, which could be added to a wound bed as a method of detecting the presence of bacteria and also immobilized hydrogel materials. The latter might be used as active wound dressings. In another version of this technology hyperbranched polymers will be synthesized to have peptide chain ends that can be degraded by specific extracelluar proteases. In this instance, the polymers will detect the bacteria following degradation of the end groups, which will expose polar carboxylic end groups. Thus, these systems will progress from a tight non-solvated globule to an open solvated chain in the presence of the target bacteria. In brief, we propose to develop novel polymer technology to rapidly detect bacteria with an easily observed optical signal.

该项目将调查使用智能聚合物检测细菌的方法。这项工作的基本原理是，超支聚合物的链末端的结合可用于将聚合物连接到细菌的细胞表面上。在某些情况下，这种结合可以通过临界构象变化进行聚合物，其中聚合物在水溶液中的变化，从开放式溶剂化的线圈到紧密的非溶解球体。在其他情况下，构象变化可以在较小的温度变化结合后带来。此外，仔细设计链端配体可以用来确保可以配制结合特定于不同种类细菌的单个位点。可以通过监测位于聚合物链上的化学标签的荧光来检测从开放线圈到紧密球的变化。这些标签通过荧光变化报告了链的构象。该项目将研究两种可溶性聚合物的使用，可以将其添加到伤口床中，以检测细菌的存在以及固定的水凝胶材料。后者可以用作活跃的伤口敷料。在该技术的另一个版本中，将合成分支超支的聚合物的肽链末端，可以被特定的外ellaceluar蛋白酶降解。在这种情况下，聚合物将在末端组降解后检测细菌，这将暴露极性羧基最终基团。因此，这些系统将从靶细菌存在下从紧密的非溶解球到开放的溶剂化链。简而言之，我们建议开发新型的聚合物技术，以易于观察到的光学信号快速检测细菌。