High Performance Gel and Blot Imager

高性能凝胶和印迹成像仪

• 批准号: 7212895
• 负责人: Bruce Robert Pitt
• 金额: $ 12.56万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7212895
• 关键词: Biological; Biotechnology; Clinical Pharmacology; Color; DNA; Detection; Environmental Health; Fluorescence; Future; Gel; Image; Image Analysis; In Situ Hybridization; Individual; Institutes; Laboratories; Lasers; Medical; Medicine; Methods; Molecular; Molecular Medicine; Northern Blotting; Occupational Health; Performance; Polymerase Chain Reaction; Proteins; Public Health; Radioisotopes; Range; Regenerative Medicine; Research; Scanning; Site; Staining method; Stains; Students; Technology; Time; Training; Western Blotting; base; fundamental research; gene therapy; instrument; mRNA Differential Displays

DESCRIPTION (provided by applicant): This amended proposal is for a high performance gel and blot imager. The new instrument, General Electric-Amersham Biosciences Typhoon 9400, has three methods of detection (storage phosphor, fluorescence and chemiluminescence) and will serve as the only high sensitivity quantitative instrument for low abundance targets for multiple users in the Department of Environmental and Occupational Health (EOH), Center for Clinical Pharmacology, McGowan Institute Regenerative Medicine (MIRM) and Molecular Medicine Institute (MMI). The rationale for this request includes: a) EOH relocated to a new research (Cellomics) building at our Bridgeside Point site 1.5 miles from the main medical campus where the two current phosphoimagers are located; b) Center Clinical Pharmacology, MIRM and MMI are current occupants of the Cellomics Building and Biotechnology Center at the Bridgeside Point site and along with EOH have increased their emphasis on the utilization of molecular biological approaches that routinely require access to a broad based platform of high sensitivity and significant dynamic range; c) Typhoon 9400 will be a valuable platform for the training of pre- and postdoctoral students; d) subtle features of this instrument including 3 laser fluorescence should assure its utility in the future; and e) the short scanning time of the instrument enhances its multiuser capability by allowing more time consuming analysis to be done in individual laboratories. Applications with Typhoon 9400 include fluorescent DNA gel stain, 2-color fluorescent differential display, fluorescent protein gel stain, fluorescent multiplex PCR and Western blot analyses, imaging 2-D gels and chemiluminescence detection of proteins, phosphor imaging of radioisotopes for Northern blot and in situ hybridization. This enabling technology will advance fundamental research in environmental health sciences, regenerative medicine, clinical pharmacology and gene therapy and thereby facilitate broad based efforts in many aspects of medicine and public health.

描述（由申请人提供）：该修订提案适用于高性能凝胶和印迹成像仪。新仪器为通用电气-Amersham Biosciences Typhoon 9400，具有三种检测方法（存储磷、荧光和化学发光），将作为环境和职业健康部多个用户的唯一低丰度目标的高灵敏度定量仪器(EOH)、临床药理学中心、麦高恩再生医学研究所 (MIRM) 和分子医学研究所 (MMI)。此请求的理由包括： a) EOH 搬迁至 Bridgeside Point 站点的一座新研究 (Cellomics) 大楼，距离目前两台磷成像仪所在的主医学园区 1.5 英里； b) 临床药理学中心、MIRM 和 MMI 目前是 Bridgeside Point 细胞组学大楼和生物技术中心的占用者，并且与 EOH 一起更加重视分子生物学方法的利用，这些方法通常需要访问广泛的高基础平台灵敏度和显着的动态范围； c) 台风9400将成为博士前和博士后培养的宝贵平台； d) 该仪器的细微特征（包括 3 个激光荧光）应确保其将来的实用性； e) 仪器的扫描时间短，允许在各个实验室进行更耗时的分析，从而增强了其多用户功能。 Typhoon 9400 的应用包括荧光 DNA 凝胶染色、2 色荧光差异显示、荧光蛋白凝胶染色、荧光多重 PCR 和蛋白质印迹分析、成像 2-D 凝胶和蛋白质化学发光检测、用于 Northern 印迹和免疫印迹的放射性同位素荧光成像原位杂交。这项技术将推动环境健康科学、再生医学、临床药理学和基因治疗方面的基础研究，从而促进医学和公共卫生许多方面的广泛努力。