Research 2 Laser Analytical Flow Cytometer

研究2 激光分析流式细胞仪

• 批准号: 6730918
• 负责人: Bruce Robert Pitt
• 金额: $ 10.6万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730918
• 关键词: apoptosis; biological signal transduction; biomedical equipment purchase; flow cytometry; microbiology; stem cells; transfection

DESCRIPTION (provided by applicant): This request is for funds to purchase an analytical bench top research two-laser flow cytometer. The new instrument, a BD FACSCalibur, will serve as the only analytical cytometer in Department of Environmental and Occupational Health (EOH) of the Graduate School Public Health (GSPH) and for users in nearby Magee Women's Research Institute (MWRI) who share common interests in environmental health science and additional work in maternal/child health. The rationale for this request includes: a) The Department of Environmental and Occupational Health (EOH) is in an extensive recruitment phase with an emphasis on contemporary environmental health science that greatly increases our utilization of flow cytometry. This expansion coincides with the recent recruitment of Dr. Jerry Schatten and colleagues at our Magee Women's Research Institute (MWRI) thereby greatly further expanding our user base; b) EOH is in a newly renovated stand alone facility 0.2 to 0.4 miles from collaborating investigators (with the noted exception of much closer proximity to MWRI) requiring transit through unacclimatized outdoor routes minimizing the opportunity to share flow cytometers located in several other departments in our allied health system; c) Our major Flow Cytometry Core facility, directed by Dr. Donnenberg, relocated (September 2002) to the Hillman Cancer Center that is 1.5 miles away adding, to our need to establish an independent ability to perform analytical flow cytometry; d) The instrument is vital for training of pre- and postdoctoral students; and e) An analytical flow cytometer (e.g., FACSCalibur) will provide valuable quantitative approaches to support more complex research protocols in environmental health science with the aid of our colleagues on our advisory committee from Flow Cytometry Core (Dr. Donnenberg) and Center for Biological Imaging (Dr. Watkins). Applications will include multicolor immunophenotyping, physiological studies on transfected cells, signaling and apoptosis, stem cell biology and microbiology.

描述（由申请人提供）：此请求旨在提供资金购买一台分析台式研究型双激光流式细胞仪。新仪器 BD FACSCalibur 将作为研究生院公共卫生 (GSPH) 环境和职业健康 (EOH) 部门以及附近具有共同兴趣的 Magee 妇女研究所 (MWRI) 的用户使用的唯一分析细胞仪环境健康科学和孕产妇/儿童健康方面的其他工作。此请求的理由包括： a) 环境和职业健康部 (EOH) 正处于广泛的招聘阶段，重点是当代环境健康科学，这大大提高了我们对流式细胞术的利用率。此次扩张恰逢我们的 Magee 妇女研究所 (MWRI) 最近招募了 Jerry Schatten 博士和同事，从而大大进一步扩大了我们的用户群； b) EOH 位于新装修的独立设施中，距离合作研究人员 0.2 至 0.4 英里（值得注意的是，距离 MWRI 更近），需要通过不适应环境的户外路线进行运输，从而最大限度地减少共享位于我们其他几个部门的流式细胞仪的机会联合医疗系统； c) 我们的主要流式细胞术核心设施在 Donnenberg 博士的指导下，搬迁（2002 年 9 月）至 1.5 英里外的 Hillman 癌症中心，这增加了我们建立独立执行分析流式细胞术能力的需要； d) 该仪器对于博士前和博士后的培养至关重要； e) 在流式细胞术核心顾问委员会（Donnenberg 博士）和生物中心的同事的帮助下，分析型流式细胞仪（例如 FACSCalibur）将提供有价值的定量方法，以支持环境健康科学中更复杂的研究方案影像学（沃特金斯博士）。应用包括多色免疫表型、转染细胞的生理学研究、信号传导和细胞凋亡、干细胞生物学和微生物学。