Typhoon 9410 Variable Mode Imager

Typhoon 9410 可变模式成像仪

• 批准号: 6877534
• 负责人: John M Kyriakis
• 金额: $ 14.15万
• 依托单位: TUFTS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877534
• 关键词: bioimaging /biomedical imaging; biological signal transduction; biomedical equipment purchase; cardiovascular disorder; genetic regulation; imaging /visualization /scanning; protein quantitation /detection

DESCRIPTION (provided by applicant): The investigators of the Molecular Cardiology Research Institute (MCRI) at the New England Medical Center use in vitro molecular biological, cell biological and transgenic approaches to explore the molecular mechanisms of cardiovascular disease and to validate new therapeutic targets. MCRI investigators pursue varied, yet complementary avenues of research--the vast majority of which are NIH-supported. Two broad treas of research within the MCRI include cardiomyocyte and vascular biology. We examine the normal and pathophysiologic regulation and function of cardiomyocyte hypertrophy and apoptosis, vascular inflammation, vasomotor tone, cardiac development and cell proliferation. A large fraction of NIH-funded MCRI Investigators as well as other NIH-funded Investigators within the Tupper Research Building, wherein the MCRI is housed are, as a major focus of their work, currently examining protein phosphorylation and gene regulation. These studies rely heavily on the use of western blotting, autoradiography and microarray analysis--often within the same experiment. Moreover, much of this work involves the examination of] clinical samples which are typically available only sporadically, and in fleetingly small quantities. Similarly, [samples from transgenic animals--mice especially--provide only small amounts of starting material which "often needs to be subdivided for parallel analysis using separate pieces of equipment. Key to the success of signal transduction and gene regulation research are reagents and equipment capable of generating reproducible results at the high levels of sensitivity needed to detect and monitor low abundance macromolecular species. At this time, we are relying on individual investigators', outdated, low sensitivity equipment, which substantially reduces the efficiency, flexibility and throughput of our research. A dedicated state of the art gel/blot analyzer, equipped also for microarray analysis, with the ability to use the most up to date, high sensitivity, three color analysis methods, would substantially enhance the throughput, reproducibility and flexibility of our work. Such an instrument is not currently available to the NIH-funded investigators taking part in this proposal. The purpose of this proposal is to gain the support needed to purchase an Amersham Biosciences Typhoon 9410 Variable Mode imager that can be used for the wide array of protein phosphorylation, signal transduction and gene regulation applications that comprise the current and growing NIH-funded research base described by the investigators of this proposal.

描述（由申请人提供）：新英格兰医学中心分子心脏病学研究所（MCRI）的研究人员利用体外分子生物学、细胞生物学和转基因方法来探索心血管疾病的分子机制并验证新的治疗靶点。 MCRI 的研究人员追求多种但互补的研究途径——其中绝大多数是 NIH 支持的。 MCRI 的两大研究领域包括心肌细胞和血管生物学。我们检查心肌细胞肥大和凋亡、血管炎症、血管舒缩张力、心脏发育和细胞增殖的正常和病理生理调节和功能。大部分由 NIH 资助的 MCRI 研究人员以及 MCRI 所在的 Tupper 研究大楼内的其他 NIH 资助的研究人员目前的主要工作重点是研究蛋白质磷酸化和基因调控。这些研究很大程度上依赖于蛋白质印迹、放射自显影和微阵列分析的使用——通常在同一实验中进行。此外，这项工作的大部分涉及临床样本的检查，这些样本通常只能零星地获得，而且数量极少。同样，[来自转基因动物（尤其是小鼠）的样品仅提供少量起始材料，这些起始材料“通常需要细分以使用单独的设备进行平行分析。信号转导和基因调控研究成功的关键是试剂和能够在检测和监测低丰度大分子物种所需的高灵敏度下产生可重复结果的设备目前，我们依赖于个别研究人员的过时的低灵敏度设备，这大大降低了效率和灵活性。专用的最先进的凝胶/印迹分析仪，也适用于微阵列分析，能够使用最新的、高灵敏度的三色分析方法，将大大提高通量、重现性和我们工作的灵活性目前无法提供给参与该提案的 NIH 资助的研究人员。 该提案的目的是获得购买 Amersham Biosciences Typhoon 9410 可变模式成像仪所需的支持，该成像仪可​​用于广泛的蛋白质磷酸化、信号转导和基因调控应用，这些应用构成了当前和不断发展的 NIH 资助的研究基地该提案的研究人员对此进行了描述。