FLA5000 Imager and Progenesis Analytical Software

FLA5000 成像仪和 Progenesis 分析软件

• 批准号: 6877398
• 负责人: TENG-LEONG CHEW
• 金额: $ 15.55万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877398
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; computer program /software; proteomics

DESCRIPTION (provided by applicant): This shared instrumentation proposal brings together a diverse group of investigators from several departments at Northwestern University, who work on a great variety of models, including prokaryotes, viruses and cancer in humans and animals. Their studies encompass a wide range of cellular processes that extend from cell junctions to virus-induced cellular changes. The common focus for this group of projects is their reliance on examination of protein expression in correlation with various cellular events in transport and signaling. This application requests funds to purchase one major instrument together with an analytical software package to interface with it: the Fuji FLA-5000 imaging system and NonLinear Dynamics' Progenesis software that will enable investigators to capture and compare complex protein patterns, and isolate protein spots from 2D gels for further analysis as well as analysis of other images such as multi-well plates or 1D PAGE gels. The FLA-5000 imaging system has several features that make it uniquely suitable for a wide variety of imaging applications: large scanning size; user-selectable scanning resolutions; dynamic response range; multiple lasers; multi-format detection (radioisotope, fluorescence, chemiluminescence, and epi-illumination. Progenesis software is fast becoming the industry standard for complex pattern analysis, especially for detection of differentially expressed proteins from 2D gels. This instrument system will be centrally located in the Northwestern University Biotechnology Laboratory. This Laboratory has a well established training and technical support program, and is used by over 120 laboratories from basic and clinical departments of the Medical and other schools of Northwestern University. The purchase of FLA-5000 imager and Progenesis software will provide a state-of-the-art system that will be used by many laboratories studying a wide range of biological problems. Due to its multifunctional nature this system will enhance interactions between investigators working in diverse areas of biology and medical science.

描述（由申请人提供）：这个共享仪器提案汇集了来自西北大学多个系的不同研究人员小组，他们研究各种模型，包括原核生物、病毒以及人类和动物的癌症。他们的研究涵盖了从细胞连接到病毒诱导的细胞变化的广泛细胞过程。这组项目的共同焦点是它们依赖于检查与运输和信号转导中的各种细胞事件相关的蛋白质表达。该申请需要资金购买一台主要仪器以及与其连接的分析软件包：Fuji FLA-5000 成像系统和 NonLinear Dynamics 的 Progenesis 软件，该软件将使研究人员能够捕获和比较复杂的蛋白质模式，并从其中分离蛋白质点2D 凝胶用于进一步分析以及其他图像分析，例如多孔板或 1D PAGE 凝胶。 FLA-5000 成像系统具有多种特性，使其特别适合各种成像应用：扫描尺寸大；用户可选择的扫描分辨率；动态响应范围；多个激光器；多格式检测（放射性同位素、荧光、化学发光和落射照明。Progenesis 软件正迅速成为复杂模式分析的行业标准，特别是用于检测 2D 凝胶中差异表达的蛋白质。该仪器系统将位于西北大学的中心大学生物技术实验室。该实验室拥有完善的培训和技术支持计划，被西北大学医学院和其他学院的120多个实验室使用。 FLA-5000 成像仪和 Progenesis 软件将提供最先进的系统，许多实验室将使用该系统来研究各种生物问题，由于其多功能性，该系统将增强在不同领域工作的研究人员之间的互动。生物学和医学。