Functional Analysis of the Mesolimbic Dopamine System

中脑边缘多巴胺系统的功能分析

• 批准号: 7456479
• 负责人: LESLIE A SOMBERS
• 金额: $ 3.69万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-11-01 至 2008-07-18
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7456479
• 关键词: Area; Body Regions; Brain; Characteristics; Chemicals; Chromosome Pairing; Cocaine; Data; Data Files; Detection; Dimensions; Disease regression; Distant; Dopamine; Dopaminergic Cell; Drug abuse; Dual Data Collection; Electrodes; Electrophysiology (science); Environment; Event; Fellowship; Fire - disasters; Individual; Infusion procedures; Intravenous; Investigation; Length; Life; Localized; Location; Measurement; Measures; Methodology; Microelectrodes; Monitor; Names; Neurobiology; Neurons; Nucleus Accumbens; Pathway interactions; Personal Satisfaction; Pharmaceutical Preparations; Physiologic pulse; Placement; Procedures; Process; Pulse taking; Rate; Rattus; Relative (related person); Rewards; Sampling; Scanning; Scientist; Signal Transduction; Surface; Synapses; System; Techniques; Technology; Time; Ventral Tegmental Area; Work; analytical method; brain tissue; carbon fiber; density; drug of abuse; extracellular; improved; mesolimbic system; neuronal circuitry; postsynaptic; presynaptic; relating to nervous system; response; sensor; size; transmission process

The mesolimbic dopamine projection from the ventral tegmental area (VTA) to the nucleus accumbens (NAc) has been implicated in the neurobiological actions of many drugs of abuse. However, the complexity of this system presents a significant barrier to investigation of its function. Thus, scientists have studied its components in fragments. In the proposed work, fast-scan cyclic voltammetry will be used to measure subsecond changes in dopamine concentration in the NAc with dual channel extracellular electrophysiology recordings of neuronal activity taken simultaneously in both the NAc and VTA. The electrochemical data will be analyzed with principal component regression, allowing dopamine concentration changes to be objectively distinguished from other electrochemical contributors and selectively quantitated. This combined approach will allow for the determination of the precise relationship between rapid dopamine release and the electrical activity at both ends of this pathway. The size of the probe in the NAc will be optimized to provide information on the geometry of the synaptic ensembles being sampled. Once characterized the methodology will be used to investigate the effects of cocaine on this reward-related brain circuitry.

中脑边缘多巴胺从腹侧被盖区（VTA）到伏隔核（NAc）的投射 与许多滥用药物的神经生物学作用有关。然而，这个问题的复杂性 系统对研究其功能构成了重大障碍。于是，科学家们研究了它的 片段中的组件。在拟议的工作中，快速扫描循环伏安法将用于测量 通过双通道细胞外电生理学观察 NAc 中多巴胺浓度的亚秒级变化 NAc 和 VTA 中同时记录的神经元活动。电化学数据将 通过主成分回归进行分析，允许多巴胺浓度变化 与其他电化学贡献者客观区分并选择性定量。这结合了 该方法将能够确定快速多巴胺释放与 该通路两端的电活动。 NAc 中探针的尺寸将被优化以提供 有关被采样的突触集合的几何形状的信息。一旦表征 该方法将用于研究可卡因对这种与奖励相关的大脑回路的影响。