POPULATION GENETICS OF NATIVE AMERICAN TRIBES

美洲原住民部落的人口遗传学

• 批准号: 2565416
• 负责人: J LONG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2565416
• 关键词: European; Native Americans; alleles; clinical research; culture; gene frequency; human genetic material tag; human population genetics; human subject; human tissue; language; linkage mapping

The purpose of this work is to ascertain the numbers of alleles, allele frequencies, and allele frequency differences among American Indian tribes. The genetic systems being typed are the same as those being used currently in our genetic linkage analyses. Approximately 30 individuals from each of 20 tribes collected primarily at the Albuquerque Indian Hospital are being analyzed. Various tests for allele frequency differences between tribal groupings based on cultural and linguistic affinities are being performed. This information is important to genetic linkage and disease association studies on Americans Indians because poor estimates of allele frequencies can result in false evidence for linkage, and allelic heterogeneity among groups can create spurious associations with disease. In order to more fully quantify isolate structure, and exploit such populations for linkage analyses, we have developed a maximum likelihood method to characterize populations by their levels of gene identity. We have applied this method to microsatellite typings for three American Indian and three European populations. The most cosmopolitan population, Maryland, had the lowest gene identity, 26% (= 74% heterozygosity). Low gene identity was also observed in Finns and Swedes, 29% each (=71% heterozygosity). By contrast, gene identity was higher in all American Indian populations 39% (=61% heterozygosity). We also find that while the overall level of gene identity does not vary much between tribes, there are significant differences in allele frequencies. Because of the inter-tribal differences in allele frequencies, it is important that linkage analysis in American Indians proceeds with allele frequencies specific for the tribe from which the pedigrees were sampled.

这项工作的目的是确定等位基因的数量 美洲印第安人的频率和等位基因频率差异 部落。键入的遗传系统与所使用的遗传系统相同 目前正在我们的遗传联系分析中。大约30个人 主要是在阿尔伯克基印第安人收集的20个部落中的每个部落中的每个部落 正在分析医院。 等位基因频率的各种测试 基于文化和语言的部落分组之间的差异 正在执行亲和力。 此信息对遗传很重要 连锁与疾病协会对美国人印第安人的研究，因为很差 等位基因频率的估计可能会导致链接的错误证据， 组之间的等位基因异质性可以建立虚假的关联 疾病。 为了更充分地量化分离株结构，并 利用此类人群进行联系分析，我们已经开发了一个 最大似然方法来表征人群的水平 基因身份。 我们已经将此方法应用于微卫星输入 三个美洲印第安人和三个欧洲人口。最多 马里兰州的大都市人口具有最低的基因身份，26％（= 74％杂合性）。 在芬兰人中也观察到低基因身份 瑞典人，每人29％（= 71％的杂合性）。 相比之下，基因身份是 所有美洲印第安人人口较高39％（= 61％的杂合性）。我们 还发现，尽管基因身份的总体水平不变 部落之间很大程度上，等位基因存在显着差异 频率。由于等位基因的部落间差异 频率，重要的是在美洲印第安人中进行连锁分析 收益的等位基因频率特定于部落 采样了谱系。