GENE MAPPING AND LINKAGE STUDIES WITH SHORT TANDEM REPEAT (STR) MARKERS

短串联重复 (STR) 标记的基因作图和连锁研究

• 批准号: 3745197
• 负责人: J LONG
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3745197
• 关键词: alcoholism /alcohol abuse; alleles; behavioral genetics; computer program /software; disease /disorder proneness /risk; fluorescent dye /probe; genetic markers; genetic polymorphism; genotype; human genetic material tag; human subject; linkage mapping; method development; nucleic acid repetitive sequence; polymerase chain reaction

We are searching for genetic linkage between short tandem repeat (STR) markers and genes that contribute to the predisposition to alcoholism and related behaviors. This involves development of a battery of 200 highly polymorphic marker loci that span the human genome at approximately 10-15 centimorgan intervals. We are using florescent dye technology and constructing panels of 10-20 loci that can be run simultaneously in one gel lane. This will facilitate fast and accurate data collection. Computer programs are being written to interface between the gel analysis machinery and a statistical database. In addition, we are developing methods for PCR-STR optimization and for precise STR allele determinations. As more genotypes are collected in the next year, we will begin linkage analyses using sequential likelihood and affected relative pair methods. The informativeness of STR markers bestows new and heretofore unattainable power for establishing, and excluding, genetic linkage between definable chromosome segments and genetic vulnerability to disease.

我们正在寻找短串联重复（STR）之间的遗传连接 有助于酒精中毒的标记和基因 相关行为。这涉及开发200个电池 跨越人类基因组大约10-15的多态性标记基因座 厘米间隔。我们正在使用荧光染料技术和 构造10-20个基因座的面板，可以同时运行 凝胶巷。这将有助于快速准确的数据收集。电脑 程序正在写入凝胶分析机械之间的接口 和一个统计数据库。此外，我们正在开发用于 PCR-STR优化和精确的STR等位基因确定。更多 基因型是在明年收集的，我们将开始链接分析 使用顺序的可能性和受影响的相对对方法。这 STR标记的信息性赋予了新的和迄今无法实现的 建立和排除可定义之间的遗传联系的能力 染色体段和遗传易受疾病的脆弱性。