Epi-fluorescence array microscope

落射荧光阵列显微镜

• 批准号: 7110011
• 负责人: CHEN LIANG
• 金额: $ 10.79万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7110011
• 关键词: fluorescence; lens; performance

DESCRIPTION (provided by applicant): DMetrix is developing a line of innovative array-microscope slide scanner instruments to generate virtual slides. Our core technology is an array of miniature objectives, each measuring less than 2 mm in diameter and 7 mm in length, slightly staggered in the array, to produce a single, seamless sweep of a microscope slide. The array microscope concept relies on imaging in parallel in order to significantly reduce the time necessary to acquire digital, high-resolution microscope-slide images. We propose here to develop an epi-fluorescence configuration of the array-microscope concept. The proposed epi-fluorescence array-microscope system will include 100 miniature microscope objectives designed and optimized for fluorescence imaging by DMetrix. This array microscope will be based on a novel "many-to-one" optics configuration that allows efficient and simultaneous illumination of the 100 miniature objectives' fields of view. The long-term goal is to create a high-throughput, highly automated fluorescence microscope. The array-microscope virtual-slide scanner will allow digitization of fluorescently labeled specimens at rates at least 10 times faster than conventional single-optical-axis optical systems. Applications of such an epi-fluorescence virtual slide scanner are to be found in molecular pathology, pharmaceutical research, case archiving, and telepathology. The high scanning speed of the proposed instrument will enable either a high throughput measured in slides/day or the acquisition of multi-layer ("z- stack") and/or multi-spectral image sets in minutes per slide.

描述（由申请人提供）：DMetrix 正在开发一系列创新型阵列显微镜载玻片扫描仪仪器来生成虚拟载玻片。我们的核心技术是一系列微型物镜，每个物镜的直径小于 2 毫米，长度小于 7 毫米，在阵列中稍微交错，以产生显微镜载玻片的单一、无缝扫描。阵列显微镜概念依赖于并行成像，以显着减少获取数字高分辨率显微镜载玻片图像所需的时间。我们在此建议开发阵列显微镜概念的落射荧光配置。拟议的落射荧光阵列显微镜系统将包括 100 个为 DMetrix 荧光成像而设计和优化的微型显微镜物镜。该阵列显微镜将基于一种新颖的“多对一”光学配置，可以高效、同时照明 100 个微型物镜的视场。长期目标是制造高通量、高度自动化的荧光显微镜。阵列显微镜虚拟载玻片扫描仪能够以比传统单光轴光学系统快至少 10 倍的速度对荧光标记样本进行数字化。这种落射荧光虚拟载玻片扫描仪的应用可在分子病理学、药物研究、病例存档和远程病理学中找到。所提出的仪器的高扫描速度将实现以载玻片/天测量的高通量或在每张载玻片几分钟内采集多层（“z-堆栈”）和/或多光谱图像集。