A Zeiss LSM800 confocal microscope with Airyscan

配备 Airyscan 的 Zeiss LSM800 共焦显微镜

• 批准号: 9075249
• 负责人: Dennis Brown
• 金额: $ 37.37万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075249
• 关键词: 14 year old; Cells; Communities; Confocal Microscopy; Core Facility; Ensure; Fluorescence Resonance Energy Transfer; Funding; General Hospitals; Health; Housing; Image; Imaging Techniques; Individual; Institution; Leadership; Life; Massachusetts; Medical Research; Membrane Biology; Microscope; Microscopy; Peer Review; Preparation; Publications; Research; Resolution; Services; Specimen; System; Techniques; Time; United States National Institutes of Health; base; detector; fluorescence imaging; imaging platform; improved; instrument; live cell imaging; member; microscopic imaging; multidisciplinary; programs; ranpirnase; repaired; research study

 DESCRIPTION (provided by applicant): We request funds to purchase a Zeiss LSM800 Confocal Microscope system with Airyscan to replace our 14 year old Zeiss/BioRad Radiance system that Zeiss can no longer guarantee to support or repair. The new instrument will be housed within the Program in Membrane Biology (PMB) Microscopy Core at the Massachusetts General Hospital (MGH) in the multidisciplinary Simches Research Center building. The mandate of this MGH approved Core facility is to provide access to a full range of techniques using microscopy and imaging to 2 NIH funded P30 Centers, Thematic Center members in the Simches building, and individual research groups within the MGH community and beyond. The PMB Core has developed considerable expertise in static and live cell imaging techniques that that been used by our extensive user base over the years. Our sophisticated imaging capabilities were enhanced 4 years ago by the incorporation of a Nikon A1R confocal platform to function alongside our older Zeiss Radiance, and together these instruments have been able to provide sufficient imaging bandwidth to serve our users. However, the Nikon is extensively used for longer term, live cell imaging, including FRET and FRAP experiments on live cells, while many of our users require instead consistent and timely access to a simpler confocal to perform more basic static imaging of fixed specimens. Unfortunately, we can no longer promise our many users that such access will continue after any given point in time, based on the fact that Zeiss can no longer guarantee to repair the Radiance. Therefore, the purpose of this application is: 1) To ensure that we can continue to provide routine confocal microscopy services to our extensive Core user base in the face of an old and potentially non repairable existing system; 2) To expand our Core capabilities to provide higher sensitivity and higher resolution (via the Airyscan detector) to our user base that will expand their imaging capabilities for exploring cellular details more precisely. The Airyscan detector provides a remarkable increase in resolution that is achieved easily and without the expense and specimen preparation issues that are associated with many other super resolution techniques. Since the PMB Core was founded in 1986, it has become a central part of the MGH/HMS medical research community. Over 37 NIH funded PIs and their numerous lab members within the HMS/MGH and in other institutions made significant user of the Core confocal in the past 12 months alone, and we have supported literally hundreds of peer review publications over the years. The Core has an ongoing quest to constantly improve and upgrade Core facilities in support of its user base. This has been achieved using funds from NIH SIG applications as well as considerable institutional support from the MGH leadership. Providing our Core users with continued access to an up to date fluorescence imaging platform is essential to maintaining the MGH community at the cutting edge of NIH funded, health related research.

 描述（由适用提供）：我们要求资金使用Airyscan购买Zeiss LSM800共聚焦显微镜系统，以替换我们14岁的Zeiss/Biorad辐射系统，Zeiss无法再保证支持或维修。该新仪器将在马萨诸塞州综合医院（MGH）的膜生物学（PMB）显微镜核心核心中安置在多学科SIMCHES研究中心大楼中。该MGH批准的核心设施的任务是，使用显微镜和成像为2个NIH资助的P30中心，Simches大楼的主题中心成员以及MGH社区及其他地区的单个研究小组提供全方位技术的访问。 PMB核心在静态和活细胞成像技术方面开发了丰富的专业知识，这些技术多年来一直在我们广泛的用户群中使用。尼康A1R共聚焦平台的公司在4年前增强了我们复杂的成像功能，可与我们的较旧的Zeiss辐射一起运行，并且这些乐器共同提供了足够的成像带宽来为我们的用户提供服务。但是，尼康被广泛用于长期，活细胞成像，包括在活细胞上进行的FRET和FRAP实验，而我们的许多用户则需要一致且及时访问更简单的共聚焦以执行固定样品的更基本的静态成像。不幸的是，我们无法再向许多用户保证，基于蔡司无法再保证修复光芒的事实，这种访问将在任何给定时间点之后继续下去。因此，本应用程序的目的是：1）确保我们可以在面对旧的且可能不可修理的现有系统的情况下向我们广泛的核心用户群提供常规共聚焦显微镜服务； 2）扩大我们的核心功能，以提供更高的灵敏度和更高的分辨率（通过Airyscan检测器），以扩大其成像功能 更精确地探索细胞细节。 Airyscan探测器提供了显着的分辨率，可以轻松实现，而没有与许多其他超级分辨率技术相关的费用和标本准备问题。自从1986年成立PMB核心以来，它已成为MGH/HMS医学研究界的核心部分。仅在过去的12个月中，超过37个NIH资助了HMS/MGH内的PIS及其众多实验室成员，在其他机构中，PIS及其核心共享的重要用户，多年来，我们几乎支持了数百个同行评审出版物。核心正在持续不断改善和升级核心设施以支持其用户群。这是使用NIH SIG应用程序的资金以及MGH领导层的可考虑机构支持来实现的。为我们的核心用户提供持续访问最新的荧光成像平台，对于维持NIH资助的与健康相关研究的最前沿的MGH社区至关重要。