Array-microscope Multispectral Whole Slide Imager

阵列显微镜多光谱全玻片成像仪

• 批准号: 7154317
• 负责人: CHEN LIANG
• 金额: $ 10.6万
• 依托单位: DMETRIX, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-15 至 2008-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7154317
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer assisted diagnosis; cytodiagnosis; high throughput technology; microarray technology; microspectrophotometry; miniature biomedical equipment; molecular /cellular imaging; optical tomography; prognosis; sectioning; tissue /cell preparation

DESCRIPTION (provided by applicant): Project Summary/Abstract: We propose to develop an innovative "many-to-one" optical system for a whole-slide multispectral slide scanner to rapidly generate virtual slides in pathology. A primary use of this optical system is to enable multispectral imaging of tissue and tissue-microarray slides. Multispectral imaging is a key development in advancing diagnostic and prognostic tissue-based pathology analysis. Multispectral imaging has its roots in remote sensing. In the context of microscopy, it is still a recent development. The main advantage of multispectral imaging, in remote sensing as well as in microscopy, is the digital unmixing of spectrally overlapping mineral or molecular signatures, respectively. The optical system proposed here will allow scanning a microscope slide in a single sweep at a numerical aperture of NA = 0.65, covering the entire width and length of a slide with 0.47-um sampling. In addition, the proposed multispectral slide scanner will deliver at least 16 contiguous spectral-band measurements at each (0.47 um)2 pixel in the whole-slide image. All multispectral data will be collected in 10's of seconds, 5 or more times faster than existing whole-slide scanners which only capture 3 colors per specimen. At this time, there is no equivalent product on the market. The Phase I project emphasizes design and assembly of an instrument prototype. The evaluation of the prototype emphasizes image quality, optical transfer efficiency, and accuracy of collected multispectral data. Measurements taken with the prototype will be compared for consistency with multispectral-imaging results obtained by our 2 collaborators. In Phase II, we plan to develop a modular, "industrial" array-microscope instrument for rapid multispectral imaging, applicable in brightfield and epi-fluorescence imaging modalities. Project Narrative: Multispectral technology, combined with Dmetrix's ultra-rapid, automated imaging technology, represents a key development in advancing diagnostic and prognostic tissue-based pathology analysis. Multispectral imaging technology will be used by the surgical pathologist to define tumor etiology, multiparameter assays and prognostics as well as therapeutic drug decisions.

描述（由申请人提供）： 项目摘要/摘要：我们建议开发一种创新的“多对一”光学系统，用于全玻片多光谱玻片扫描仪，以快速生成病理学中的虚拟玻片。该光学系统的主要用途是实现组织和组织微阵列载玻片的多光谱成像。多光谱成像是推进基于组织的病理学分析的诊断和预后的关键发展。多光谱成像起源于遥感。在显微镜的背景下，它仍然是一个新的发展。多光谱成像在遥感和显微镜中的主要优点是分别对光谱重叠的矿物或分子特征进行数字分离。这里提出的光学系统将允许以 NA = 0.65 的数值孔径单次扫描显微镜载玻片，以 0.47 微米采样覆盖载玻片的整个宽度和长度。此外，所提出的多光谱幻灯片扫描仪将在整个幻灯片图像中的每个 (0.47 um)2 像素处提供至少 16 个连续光谱带测量。所有多光谱数据将在 10 秒内收集完毕，比现有的全玻片扫描仪（每个样本仅捕获 3 种颜色）快 5 倍或更多。目前，市场上还没有同等产品。一期项目重点是仪器原型的设计和组装。原型的评估强调图像质量、光学传输效率和收集的多光谱数据的准确性。使用原型进行的测量将与我们的 2 位合作者获得的多光谱成像结果进行比较，以确保一致性。在第二阶段，我们计划开发一种模块化的“工业”阵列显微镜仪器，用于快速多光谱成像，适用于明场和落射荧光成像模式。项目叙述：多光谱技术与 Dmetrix 的超快速自动成像技术相结合，代表了推进基于组织的病理学分析的诊断和预后的关键发展。外科病理学家将使用多光谱成像技术来定义肿瘤病因、多参数分析和预后以及治疗药物决策。