Rodents with genetic differences in alcohol preference

酒精偏好存在遗传差异的啮齿类动物

基本信息

批准号：
7613268
负责人：
LAWRENCE LUMENG
金额：
$ 9.78万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-07-01 至 2010-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7613268
关键词：
Adolescent Alcohol abuse Alcohol consumption Alcoholism Alcohols Animal Model Animals Award Behavior Behavioral Breeding Candidate Disease Gene Characteristics Charge Chromosome Mapping Communities Condition Data Development Ensure Exhibits Family history of Funding Funding Agency Generations Genes Genetic Genetic Code Genetic Crosses Genome Goals Grant Human Inbred Strain Indiana Individual Institution Knowledge Mediating Medicine Modeling Mus Neurobiology Numbers Phenotype Price Production Program Research Project Grants Property Protocols documentation Publishing Rattus Rattus norvegicus Research Research Personnel Resource Sharing Resources Rodent Scientist Screening procedure Standards of Weights and Measures Structure Time Universities Upper arm alcohol abuse therapy alcohol effect alcohol relapse alcohol research alcoholism/alcohol abuse analog craving deprivation drinking human APEX1 protein human disease improved neurobiological mechanism pre-clinical preference problem drinker progenitor programs

项目摘要

Rats and mice as models for human disease have made invaluable contributions to almost every field of human medicine - alcoholism included. Recently, scientists have published nearly the entire genome of the common rat, making it possible to compare it to the genetic maps of mice and humans, and to improve the efficiency of candidate gene identification. This major advance is expected to yield important clues into the genetics and neurobiology of alcohol abuse and alcoholism. We have raised by selective breeding the P/NP and HAD/LAD (replicate) rat lines and the HAP/LAP (replicate) mouse lines that exhibit divergent alcohol preference. We have also raised inbred strains of P/NP, HAD1/LAD1 and HAD2/LAD2 which will be useful for performing genetic crosses and intercrosses. These selected rat and mouse lines and inbreds constitute a unique, already established, research resource. The P rats fulfill all of the criteria of an animal model of alcoholism. By contrast, due to incomplete characterization, the HAD rats and the HAP mice at this time only satisfy some of the criteria. The P and HAD rats are also excellent animal models to study alcohol relapse or craving (they display the alcohol deprivation effect, ADE), to study adolescent alcohol abuse, and for medicinal development in the treatment of alcoholism. The overall goal of this application is to establish an Alcohol Research Resource Center so that these selectively bred and inbred lines/strains can be widely shared by alcohol researchers at both Indiana University and with many off-campus investigators in external research and academic institutions. The budgetary charge for these rats will be reduced from the current charge structure established by our Indiana Alcohol Research Center. The specific aims of this application includes: (1) To continue selective breeding of the replicate HAD/LAD lines of rats. (2) To produce and export to off-campus researchers selectively bred or inbred P/NP, HAD1/LAD1 and HAD2/LAD2 rats. And (3) To produce and export to external institutions selectively bred HAP1/LAP1 and HAP2/LAP2 mice.

大鼠和小鼠作为人类疾病的模型已为几乎每个领域的每个领域做出了宝贵的贡献人类医学 - 包括酒精中毒。最近，科学家已经发表了几乎整个基因组常见的大鼠，可以将其与小鼠和人类的遗传图进行比较，并改善候选基因鉴定的效率。预计这一重大进展将为您带来重要的线索酒精滥用和酒精中毒的遗传学和神经生物学。我们通过选择性繁殖P/NP提出了并具有/lad（重复）大鼠线和表现出不同酒精的HAP/LAP（重复）小鼠系偏爱。我们还提高了p/np的近交菌株，have是1/lad1和had2/lad2，这将很有用进行遗传杂交和间交叉。这些选定的大鼠和小鼠线以及近交构成一种独特的，已经建立的研究资源。 P大鼠满足动物模型的所有标准酗酒。相比之下，由于表征不完整，目前有大鼠和HAP小鼠只能满足一些标准。 P和Hav的大鼠也是研究酒精的出色动物模型复发或渴望（它们显示出贫困效果，ADE），研究青少年酒精滥用和用于治疗酒精中毒的药物发展。该应用程序的总体目标是建立酒精研究资源中心，以便这些有选择的育种和近交系列/菌株可以广泛印第安纳大学的酒精研究人员和许多外部的校外调查员共享研究和学术机构。这些大鼠的预算收费将从当前减少我们的印第安纳酒精研究中心建立的电荷结构。此应用程序的具体目的包括：（1）继续选择性繁殖，重复育种大鼠的lad线。（2）生产和出口到校外研究人员有选择地育种或近交P/NP，HAD1/LAD1和HAD2/LAD2大鼠。和（3）生产并出口到外部机构，有选择地繁殖HAP1/LAP1和HAP2/LAP2小鼠。