Rodents with genetic differences in alcohol preference

酒精偏好存在遗传差异的啮齿类动物

• 批准号: 7253447
• 负责人: LAWRENCE LUMENG
• 金额: $ 39.38万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7253447
• 关键词: Adolescent; Alcohol abuse; Alcohol consumption; Alcoholism; Alcohols; Animal Model; Animals; Award; Behavior; Behavioral; Breeding; Candidate Disease Gene; Characteristics; Charge; Chromosome Mapping; Communities; Condition; Data; Development; Ensure; Exhibits; Family history of; Funding; Funding Agency; Generations; Genes; Genetic; Genetic Code; Genetic Crosses; Genome; Goals; Grant; Human; Inbred Strain; Indiana; Individual; Institution; Knowledge; Mediating; Medicine; Modeling; Mus; Neurobiology; Numbers; Phenotype; Price; Production; Program Research Project Grants; Property; Protocols documentation; Publishing; Rattus; Rattus norvegicus; Research; Research Personnel; Resource Sharing; Resources; Rodent; Scientist; Screening procedure; Standards of Weights and Measures; Structure; Time; Universities; Upper arm; alcohol abuse therapy; alcohol effect; alcohol relapse; alcohol research; alcoholism/alcohol abuse; analog; craving; deprivation; drinking; human APEX1 protein; human disease; improved; neurobiological mechanism; pre-clinical; preference; problem drinker; progenitor; programs

DESCRIPTION (provided by applicant): Rats and mice as models for human disease have made invaluable contributions to almost every field of human medicine - alcoholism included. Recently, scientists have published nearly the entire genome of the common rat, making it possible to compare it to the genetic maps of mice and humans, and to improve the efficiency of candidate gene identification. This major advance is expected to yield important clues into the genetics and neurobiology of alcohol abuse and alcoholism. We have raised by selective breeding the P/NP and HAD/LAD (replicate) rat lines and the HAP/LAP (replicate) mouse lines that exhibit divergent alcohol preference. We have also raised inbred strains of P/NP, HAD1/LAD1 and HAD2/LAD2 which will be useful for performing genetic crosses and intercrosses. These selected rat and mouse lines and inbreeds constitute a unique, already established, research resource. The P rats fulfill all of the criteria of an animal model of alcoholism. By contrast, due to incomplete characterization, the HAD rats and the HAP mice at this time only satisfy some of the criteria. The P and HAD rats are also excellent animal models to study alcohol relapse or craving (they display the alcohol deprivation effect, ADE), to study adolescent alcohol abuse, and for medicinal development in the treatment of alcoholism. The overall goal of this application is to establish an Alcohol Research Resource Center so that these selectively bred and inbred lines/strains can be widely shared by alcohol researchers at both Indiana University and with many off-campus investigators in external research and academic institutions. The budgetary charge for these rats will be reduced from the current charge structure established by our Indiana Alcohol Research Center. The specific aims of this application includes: (1) to continue selective breeding of the replicate HAD/LAD lines of rats. (2) To produce and export to off-campus researchers selectively bred or inbred P/NP, HAD1/LAD1 and HAD2/LAD2 rats. And (3) to produce and export to external institutions selectively bred HAP1/LAP1 and HAP2/LAP2 mice.

描述（由申请人提供）：大鼠和小鼠作为人类疾病的模型为几乎每个人类医学领域（包括酒精中毒）做出了无价的贡献。最近，科学家几乎发表了普通大鼠的整个基因组，使其与小鼠和人类的遗传图进行比较，并提高候选基因鉴定的效率。预计这一重大进展将为酒精滥用和酒精中毒的遗传学和神经生物学提供重要的线索。我们通过选择性繁殖P/NP并具有/LAD（重复）大鼠系和hap/lap（复制）小鼠系列而提高了，这些小鼠曲线表现出不同的酒精偏爱。我们还提高了P/NP，HAD1/LAD1和HAD2/LAD2的近交性菌株，这对于执行遗传杂交和间交叉非常有用。这些选定的老鼠和鼠标线以及近交构成了一种独特的，已经建立的研究资源。 P大鼠符合酒精中毒动物模型的所有标准。相比之下，由于表征不完整，目前有大鼠和HAP小鼠只能满足某些标准。 P和有大鼠也是研究酒精复发或渴望的出色动物模型（它们表现出酒精剥夺效果，ADE），研究青少年酒精滥用以及用于治疗酒精中毒的药物发育。该应用程序的总体目的是建立酒精研究资源中心，以便印第安纳大学的酒精研究人员以及许多外部研究和学术机构的校外研究人员可以广泛地共享这些有选择的繁殖和近交系列/菌株。这些大鼠的预算收费将从我们的印第安纳酒精研究中心建立的当前收费结构中降低。该应用程序的具体目的包括：（1）继续选择性育种大鼠的LAD/LAD线。 （2）生产并出口到校外研究人员有选择地育种或近交P/NP，有1/LAD1和HAD2/LAD2大鼠。 （3）生产和出口到外部机构，有选择地育成HAP1/LAP1和HAP2/LAP2小鼠。