Combined OCT/FLIM Biochemical and Anatomical Imaging for Early Cancer Diagnosis

结合 OCT/FLIM 生化和解剖成像进行早期癌症诊断

• 批准号: 7540319
• 负责人: Javier Antonio Jo
• 金额: $ 33.46万
• 依托单位: TEXAS ENGINEERING EXPERIMENT STATION
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7540319
• 关键词: Address; Algorithms; American Cancer Society; Biochemical; Biopsy; Cancerous; Characteristics; Cheek structure; Classification; Computer Systems Development; Computing Methodologies; Contrast Media; Data; Databases; Depth; Detection; Development; Diagnosis; Diagnostic; Early Diagnosis; Epithelial; Epithelium; Fluorescence; Fluorescence Spectroscopy; Future; Goals; Hamsters; Histopathology; Image; Image Analysis; Imaging Techniques; Imaging technology; Invasive; Lesion; Malignant - descriptor; Malignant Neoplasms; Measures; Metric; Microanatomy; Modality; Modeling; Monitor; Morbidity - disease rate; Mouth Neoplasms; Multimodal Imaging; Neoplasms; Normal tissue morphology; Operative Surgical Procedures; Optical Biopsy; Optical Coherence Tomography; Optics; Oral; Oral Diagnosis; Oral cavity; Penetration; Pharyngeal structure; Premalignant; Public Health; Quality of life; Quantitative Evaluations; Range; Reporting; Research; Resolution; Screening for Oral Cancer; Screening procedure; Sensitivity and Specificity; Signal Transduction; Staging; Standards of Weights and Measures; Survival Rate; Survivors; System; Techniques; Texture; Therapeutic; Therapeutic Intervention; Time; Tissues; Visual; Work; cancer diagnosis; carcinogenesis; design; fluorescence imaging; image processing; improved; in vivo; instrumentation; malignant mouth neoplasm; molecular imaging; mortality; neoplastic; novel; novel therapeutics; optical imaging; response; size; success; tomography; tool

DESCRIPTION (provided by applicant): Early detection of neoplastic changes in the oral cavity holds great promise for improving survival rates and the quality of life of survivors. The high spatial resolution of optical imaging could make possible the diagnosis of neoplastic lesions while they are still in the submillimeter range in size and highly treatable. Both biochemical and morphological changes accompany the transition from normal tissue to neoplastic tissue. Yet the optical techniques heretofore investigated as potential screening and diagnostic tools for cancer have largely concentrated on detecting one or the other, principally because of the fundamental lack of optical imaging modalities which readily provide both types of information. Here we propose to simultaneously collect morphological and biochemical information by combining two well developed optical imaging techniques, optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM). Since OCT and FLIM measure different tissue characteristics (structural and biochemical, respectively), we hypothesize that their synergy will increase the sensitivity and specificity for detecting early epithelial cancer, compared to each modality applied independently. The ultimate goal of this line of research is to develop a novel optical imaging system and associated algorithms for noninvasive optical biopsy in oral cancer. Such a system may serve as a diagnostic and monitoring tool, a screening tool, a guide to standard biopsy and surgical intervention, and a tool for quantitative evaluation of therapeutic response. While this proposal is focused on the early detection of oral cancer we believe that the system and algorithms developed will also be generally applicable to many of the cancers of the epithelium. The following three specific aims will be addressed in this proposal: Aim 1: To develop a novel optical multimodality imaging system by combining OCT and FLIM technology for simultaneous micro-anatomical and biochemical imaging of tissue in vivo and ex vivo. Aim 2: To acquire a unique database of OCT/FLIM images and corresponding histopathology of the development of oral cancer in a hamster cheek pouch model. Aim 3: To develop new computational methods for OCT/FLIM image analysis and evaluate the sensitivity/specificity of the proposed multimodality imaging system for the early detection of oral neoplasia. The successful completion of this work will demonstrate the potential of combining OCT and FLIM as a means for non-invasive optical detection of oral pre-malignant and malignant lesions. PUBLIC HEALTH RELEVANCE: Early detection of epithelial oral cancer could significantly reduce cancer morbidity and mortality. Currently, the diagnosis of oral epithelial cancers is performed through direct or endoscopic visual inspection followed by biopsy. Since visual inspection is subjective and early pre-cancers are often not detectable under visual inspection, multiple biopsies have to be taken to increase the likelihood of finding a cancerous lesion. Therefore, techniques that can diagnose oral epithelial pre-cancer and cancer with higher accuracy than visual inspection alone are needed to guide tissue biopsy. Optical imaging is particularly well suited for imaging many of the epithelial tissues of the body including the oral cavity. Both biochemical and morphological changes accompany the transition from normal tissue to neoplastic tissue, yet the optical techniques heretofore investigated as potential screening and diagnostic tools for cancer have largely concentrated on one or the other. Here we propose to simultaneously collect both morphological and biochemical information by combining two well developed optical imaging techniques, optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM). The ultimate goal of this line of research is to develop a novel optical imaging system and associated algorithms which will enable noninvasive optical biopsy in oral cancer.

描述（由申请人提供）：口腔中的肿瘤变化的早期发现具有改善生存率和幸存者生活质量的巨大希望。光学成像的高空间分辨率可以使肿瘤病变的诊断可能使肿瘤病变的尺寸范围保持在尺寸范围且高度可处理。生化和形态学变化均伴随着从正常组织到肿瘤组织的过渡。然而，作为癌症的潜在筛查和诊断工具的迄今为止，光学技术主要集中在检测一种或另一种方面，这主要是因为基本缺乏光学成像方式，很容易提供两种类型的信息。在这里，我们建议通过结合两种发达的光学成像技术，光学相干断层扫描（OCT）和荧光寿命成像（FLIM）来同时收集形态和生化信息。由于OCT和FLIM测量了不同的组织特征（分别分别结构和生化），因此与独立应用的每种方式相比，它们的协同作用将提高检测早期上皮癌的敏感性和特异性。这一研究的最终目标是开发一种新型的光学成像系统和相关的口腔癌中非侵入性光学活检的算法。这样的系统可以用作诊断和监测工具，筛查工具，标准活检和手术干预指南以及用于定量评估治疗反应的工具。尽管该提案的重点是对口腔癌的早期发现，但我们认为，开发的系统和算法通常也适用于上皮的许多癌症。该提案将解决以下三个特定目标：目标1：通过将OCT和FLIM技术结合起来，以同时进行体内和Ex Vivo的组织的同时微动态和生化成像来开发新型的光学多模式成像系统。目标2：在仓鼠脸颊袋模型中获取OCT/FLIM图像的独特数据库以及口腔癌发展的相应组织病理学。目标3：开发新的计算方法来进行OCT/FLIM图像分析，并评估提出的多模式成像系统的灵敏度/特异性，以早期检测口腔肿瘤。这项工作的成功完成将证明将OCT和FLIM作为非侵入性光学检测前恶性病变和恶性病变的一种手段的潜力。 公共卫生相关性：早期发现上皮口腔癌可以显着降低癌症的发病率和死亡率。当前，口服上皮癌的诊断是通过直接或内镜视觉检查进行的，然后进行活检。由于视觉检查是主观的，并且在视觉检查中通常无法检测到早期的癌症，因此必须进行多次活检以增加发现癌变的可能性。因此，仅仅需要与视觉检查相比，可以使用具有更高准确性的口腔上皮前癌和癌症来指导组织活检的技术。光学成像特别适合成像人体的许多上皮组织，包括口腔。从正常组织到肿瘤组织的过渡伴随着生化和形态学变化，但是作为癌症潜在筛查和诊断工具所研究的光学技术主要集中在一个或另一个。在这里，我们建议通过结合两种发达的光学成像技术，光学相干断层扫描（OCT）和荧光寿命成像（FLIM）来同时收集形态和生化信息。这一研究线的最终目的是开发一种新型的光学成像系统和相关算法，该算法将使口腔癌中的无创光学活检。