Endogenous fluorescence lifetime endoscopy for early detection of oral cancer and dysplasia

内源性荧光终生内窥镜检查用于口腔癌和不典型增生的早期检测

基本信息

批准号：
10686883
负责人：
Javier Antonio Jo
金额：
$ 28.88万
依托单位：
UNIVERSITY OF OKLAHOMA
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-02-02 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10686883
关键词：
Adopted Algorithms American Cancer Society Benign Biological Markers Biomedical Engineering Biopsy Blinded Cancer Patient Carcinoma Characteristics Chicago Clinic Clinical Clinical Management Clinical Research Coenzymes Collaborations Collagen Data Databases Dentists Detection Diagnosis Diagnostic Drops Dysplasia Early Diagnosis Endoscopes Endoscopy Epithelium Evaluation Excision Face Fluorescence Frequencies Future Geometry Goals Health Professional Histopathology Image Imaging Device In Situ Intraepithelial Neoplasia Lesion Life Expectancy Malignant Epithelial Cell Malignant Neoplasms Metabolic Metabolism Mild Dysplasia Minor Surgical Procedures Mitochondria Modality Monitor Monitoring for Recurrence Neck Operative Surgical Procedures Optics Oral Oral Stage Oral cavity Oropharyngeal Pathologic Patient Recruitments Patients Pilot Projects Predictive Value Quality of life Recurrence Recurrent disease Reporting Research Personnel Research Project Grants Screening for Oral Cancer Screening for cancer Screening procedure Sensitivity and Specificity Specificity Speed Stains Survival Rate System Technology Testing Time Tissues Work automated algorithm biomarker panel cancer invasiveness clinically relevant computerized tools cost cost effective design diagnostic tool early screening exfoliative cytology experience fluorescence lifetime imaging image processing imaging biomarker improved in vivo in vivo fluorescence in vivo imaging machine learning algorithm malignant mouth neoplasm noninvasive diagnosis novel optical imaging oral cavity epithelium oral dysplasia oral lesion precision medicine prospective salivary assay success tool tumor tumor progression volunteer

项目摘要

The American Cancer Society estimates that 48,330 new cases of cancer in the oral cavity and pharynx will be reported this year. When diagnosed at early stages, the 5-year survival rate is 83%. However, when diagnosed at intermediate or advance stages, the 5-year survival rate drops to 62% and 38%, respectively. In addition, while early stage treatment may only require minor surgery to remove the localized tumor, later stage treatment could require surgical removal of parts of the face and neck, hence drastically reducing the patient’s quality of life. Unfortunately, benign oral lesions are sometimes difficult to distinguish from dysplasia or early invasive cancer even for healthcare professionals. As a result, only 31% of patients are diagnosed at early stages despite the fact that the oral cavity is easily accessible for direct examination. Hence, there is a critical need for new clinical technologies for reliable early diagnosis of oral cancer and dysplasia. Several screening tools for oral cancer have been commercially available, including exfoliative cytology, vital staining, salivary test, and optical interrogation; however, none of them have been demonstrated to be capable of clinically relevant sensitivity and specificity. We hypothesize that several biomarkers for oral cancer and dysplasia can be accurately quantified by endogenous fluorescence lifetime imaging (FLIM) thus enabling levels of sensitivity and specificity adequate for early detection. This bioengineering research grant focuses on developing and validating a cost-effective wide-field multispectral FLIM endoscope for noninvasive in situ detection of oral cancer and dysplasia. To that end we have developed three specific aims. Aim 1: To design and build a cost-effective multispectral FLIM endoscope for in vivo imaging of epithelial tissue in the oral cavity. Aim 2: To develop algorithms for fast and automated FLIM based early detection of oral epithelial cancer and dysplasia. Aim 3: To quantify prospectively in a pilot clinical study the capability of the proposed FLIM endoscopic tools for noninvasively early detection of oral epithelial cancer and dysplasia. The successful completion of these aims will result in a novel, accurate and cost-effective clinical tool for noninvasive in situ early detection of cancer and dysplasia. Such a tool could potentially help to improve significantly both the life expectancy and the quality of life for the more than 33,000 oral cancer patients being diagnosed each year at intermediate and advance stages. Beyond early diagnosis, this tool could also assist at every step involved on the clinical management of oral cancer patients, including treatment guidance and monitoring of disease recurrence. Finally, the demonstrated success of this clinical tool in oral epithelial cancer will herald future success with other cancers of epithelial origin, which accounts for more than 80% of all cancers.

美国癌症协会估计，今年将报告 48,330 例新的口腔和咽部癌症病例，如果在早期诊断，5 年生存率为 83%，但在中期或晚期诊断时，5 年生存率为 83%。 5年生存率分别下降至62%和38%。此外，早期治疗可能只需要进行小手术切除局部肿瘤，但后期治疗可能需要手术切除部分面部和颈部，因此。不幸的是，即使对于医疗保健专业人员来说，良性口腔病变有时也很难与不典型增生或早期浸润性癌症区分开来，因此，尽管口腔有病变，但只有 31% 的患者能够在早期得到诊断。因此，迫切需要新的临床技术来可靠地早期诊断口腔癌和发育不良，目前已经有多种口腔癌筛查工具，包括脱落细胞学、活体染色、唾液检测和口腔癌筛查。光学的然而，它们都没有被证明具有临床相关的敏感性和特异性，我们认为，口腔癌和发育不良的几种生物标志物可以通过内源性荧光寿命成像（FLIM）进行准确定量，从而实现足够的敏感性和特异性。这项生物工程研究资助的重点是开发和验证一种经济有效的广域多光谱 FLIM 内窥镜，用于口腔癌和异型增生的无创原位检测。为此，我们制定了三个具体目标。目标 1：设计和构建一种经济高效的多光谱 FLIM 内窥镜，用于口腔上皮组织的体内成像。目标 2：开发基于 FLIM 的口腔上皮癌和发育不良的快速、自动化早期检测算法。在一项试点临床研究中，前瞻性地展示了所提出的 FLIM 内窥镜工具用于无创早期检测口腔上皮癌和不典型增生的能力，这些目标的成功实现将带来一种新颖、准确和可靠的结果。用于癌症和不典型增生的非侵入性原位早期检测的具有成本效益的临床工具，这种工具可能有助于显着提高每年超过 33,000 名中期和晚期诊断的口腔癌患者的预期寿命和生活质量。除了早期诊断之外，该工具还可以协助口腔癌患者临床管理的每一步，包括治疗指导和疾病复发监测。最后，该临床工具在口腔上皮癌中所取得的成功将预示着未来的成功。与其他癌症上皮起源，占所有癌症的 80% 以上。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Multispectral autofluorescence dermoscope for skin lesion assessment.

DOI：
10.1016/j.pdpdt.2020.101704
发表时间：
2020-06
期刊：
Photodiagnosis and photodynamic therapy
影响因子：
3.3
作者：
Romano RA;Teixeira Rosa RG;Salvio AG;Jo JA;Kurachi C
通讯作者：
Kurachi C

Dual-modality optical coherence tomography and frequency-domain fluorescence lifetime imaging microscope system for intravascular imaging.

DOI：
10.1117/1.jbo.25.9.096010
发表时间：
2020-09
期刊：
Journal of biomedical optics
影响因子：
3.5
作者：
Chen X;Kim W;Serafino M;Tan Z;Jo J;Applegate B
通讯作者：
Applegate B

Discrimination of cancerous from benign pigmented skin lesions based on multispectral autofluorescence lifetime imaging dermoscopy and machine learning.

DOI：
10.1117/1.jbo.27.6.066002
发表时间：
2022-06
期刊：
JOURNAL OF BIOMEDICAL OPTICS
影响因子：
3.5
作者：
Vasanthakumari, Priyanka;Romano, Renan A.;Rosa, Ramon G. T.;Salvio, Ana G.;Yakovlev, Vladislav;Kurachi, Cristina;Hirshburg, Jason M.;Jo, Javier A.
通讯作者：
Jo, Javier A.

Intravascular optical coherence tomography method for automated detection of macrophage infiltration within atherosclerotic coronary plaques.

血管内光学相干断层扫描方法自动检测动脉粥样硬化冠状动脉斑块内的巨噬细胞浸润。

DOI：
10.1016/j.atherosclerosis.2019.09.023
发表时间：
2019
期刊：
Atherosclerosis
影响因子：
5.3
作者：
Rico-Jimenez,JoseJ;Campos-Delgado,DanielU;Buja,LMaximillan;Vela,Deborah;Jo,JavierA
通讯作者：
Jo,JavierA

Direct frequency domain fluorescence lifetime imaging using simultaneous ultraviolet and visible excitation.

使用同时紫外和可见光激发的直接频域荧光寿命成像。