PUTATIVE INDICATORS OF BIOTIN STATUS DURING MARGINAL BIOTIN DEFICIENCY

边缘生物素缺乏期间生物素状态的假定指标

• 批准号: 7377689
• 负责人: Donald M Mock
• 金额: $ 0.27万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7377689
• 关键词: PUTATIVE; INDICATORS; BIOTIN; STATUS; DURING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to accurately detect biotin deficiency, indicators of biotin status must be identified and confirmed. This is achieved through research studies in which normal subjects are made biotin deficient by feeding avidin, a protein found in raw egg white. This proposal seeks to test a new avidin source in a new outpatient study design. The specific aims of this proposal are as follows: Specific Aim #1- To determine if successful marginal biotin deficiency can be induced in normal adults by consumption of an avidin supplement with meals while subjects are followed on an outpatient basis; Specific Aim #2- To determine if the avidin-induced deficiency in an outpatient design with subjects consuming a general self-selected diet is as efficient in inducing marginal biotin deficiency as in past inpatient, diet-controlled studies in which subjects consumed an egg-white beverage with meals; Specific Aim #3- If successful induction of biotin deficiency by avidin supplementation is achieved, to assess the usefulness of the following putative indicators of biotin status: 1) lymphocyte activity of the biotin-dependent enzyme propionyl-CoA carboxylase (PCC) and calculation of PCC activation coefficients, 2) the plasma and erythrocyte content of the odd-chain fatty acids C15:0 and C17:0), and 3) 3-hydroxyisovaleric acid excretion in response to a leucine challenge; Specific Aim #4- To examine changes in urinary prostaglandins in response to biotin deficiency; Specific Aim #5- To examine changes in biotinylation of histones in response to biotin deficiency; and Specific Aim #6- To examine changes in insulin sensitivity in response to biotin deficiency. Successful completion of this study and its specific aims will confirm indices of biotin status, potentially identify new indices of biotin status, and allow other researchers an opportunity to run similar studies in an expeditious and economical manner.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。为了准确检测生物素缺乏症，必须确定并确认生物素状态的指标。这是通过研究来实现的，在研究中，通过喂食阿维丁（一种在生蛋白中发现的蛋白质），使正常受试者缺乏生物素。该建议旨在在新的门诊研究设计中测试一种新的Avidin来源。 该提案的具体目的如下：具体目的＃1-确定成功的边缘生物素缺乏症是否可以在正常成年人中通过食用Avidin补充剂，而在门诊基础上遵循受试者；具体目的＃2-确定抗原素诱导的门诊设计中的缺乏症是否是否会像过去的住院，受试者消耗鸡蛋一样，在诱发边缘生物素缺乏症的诱导边际生物素缺乏症方面有效用餐的白饮料；具体目的＃3-如果通过补充抗生物素来成功诱导生物素缺乏症，以评估生物素状态的以下推定指标的有用性：1）生物素依赖性酶的淋巴细胞活性PCC激活系数，2）奇数链脂肪酸C15：0和C17：0）的血浆和红细胞含量，以及3）3-羟基异硫化剂酸排泄，以应对leucine挑战；特定目的＃4-以响应生物素缺乏症来检查泌尿前列腺素的变化；特定目的＃5-检查对生物素缺乏的组蛋白生物素化的变化；和特定的目标＃6-以检查胰岛素敏感性的变化，以响应生物素缺乏症。 这项研究的成功完成及其具体目的将确认生物素状态的指标，可能确定生物素状态的新指数，并允许其他研究人员有机会以迅速和经济的方式进行类似的研究。