ASSESSMENT OF NUTRITIONAL STATUS OF THE WATER-SOLUBLE VITAMIN BIOTIN IN PREGNANC

水溶性维生素生物素在妊娠期的营养状况评估

• 批准号: 7377696
• 负责人: Donald M Mock
• 金额: $ 0.2万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7377696
• 关键词: ASSESSMENT; NUTRITIONAL; STATUS; WATER; SOLUBLE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Marginal biotin deficiency is quite teratogenic in some animal species. In humans, we have found that about one-third of women become marginally biotin deficient during pregnancy based on certain chemical indicators of biotin status. This proposal seeks to confirm a recently validated blood-based indicator of biotin status, activity of the biotin-dependent enzyme propionyl-CoA carboxylase (PCC), and to examine other potential indicators of biotin status during pregnancy. The specific aim of this proposal is as follows: Specific Aim #1- To test the hypothesis that a significant number of women will develop marginal biotin deficiency during normal human gestation. Biotin status will be assessed in a cross-sectional study design using lymphocyte PCC activity and activation of PCC by biotin as the primary outcome variables. Secondary outcome variables examined will include urinary excretion of biotin, urinary excretion of 3-hydroxyisovaleric acid (3HIA), urinary concentration of prostaglandins, red blood cell fatty acid accumulation, and plasma odd-chain fatty acid accumulation. Successful completion of this study and its specific aims will elucidate biotin nutrition in pregnancy and serve as a pilot study that will form the basis of an extramural grant application to further study this important question.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。边缘生物素缺乏在某些动物物种中具有相当的致畸性。在人类中，根据生物素状态的某些化学指标，我们发现大约三分之一的女性在怀孕期间略微缺乏生物素。该提案旨在确认最近验证的基于血液的生物素状态指标、生物素依赖性酶丙酰辅酶A羧化酶（PCC）的活性，并检查妊娠期间生物素状态的其他潜在指标。 该提案的具体目标如下： 具体目标#1 - 检验以下假设：大量女性在正常人类妊娠期间会出现边缘生物素缺乏症。将使用淋巴细胞 PCC 活性和生物素对 PCC 的激活作为主要结果变量，在横断面研究设计中评估生物素状态。检查的次要结果变量包括生物素的尿排泄、3-羟基异戊酸（3HIA）的尿排泄、前列腺素的尿浓度、红细胞脂肪酸积累和血浆奇数链脂肪酸积累。 这项研究的成功完成及其具体目标将阐明怀孕期间的生物素营养，并作为一项试点研究，为进一步研究这一重要问题的校外拨款申请奠定基础。