OPTICAL BIOSENSOR FOR THE EARLY DETECTION OF BREAST CANCER

用于乳腺癌早期检测的光学生物传感器

• 批准号: 7368159
• 负责人: John Ernest Shively
• 金额: $ 0.65万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7368159
• 关键词: OPTICAL; BIOSENSOR; EARLY; DETECTION; BREAST; OPTICAL; BIOSENSOR; EARLY; DETECTION; BREAST

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the first stage we propose to determine if the measurement of proteins (specifically CEA) secreted by the breast captured in a nitrocellulose filter can be used in detecting breast cancer. For that purpose we will obtain samples from nipple secretions in nitrocellulose paper from patients requiring a breast biopsy to determine the sensitivity and specificity of the assay. In a second stage an optical biosensor that has been optimized for the detection of CEA will be used to detect fluorescent labeled antibodies directed against CEA found in the serum and breast secretions. The tumor marker carcinoembryonic antigen (CEA) will be used as an early marker for breast cancer in blood and nipple secretions as a model system. Ultimately, the biosensor will be a handheld device that can be used to test minute amounts of blood or secretions in a point of service context. The availability of such a device should allow earlier and more convenient testing for CEA and, eventually, for other tumor markers.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。在第一阶段，我们建议确定在硝酸纤维素过滤器中捕获的乳腺分泌的蛋白质（特异性CEA）是否可以用于检测乳腺癌。为此，我们将从需要乳房活检以确定测定的敏感性和特异性的患者中从硝酸纤维素纸中的乳头分泌物中获取样品。在第二阶段，已优化用于检测CEA的光学生物传感器将用于检测针对血清和乳房分泌物中CEA的荧光标记的抗体。肿瘤标记癌症抗原（CEA）将用作血液和乳头分泌物中乳腺癌的早期标记作为模型系统。最终，生物传感器将是一种手持设备，可用于在服务点上测试微量的血液或分泌物。这种设备的可用性应允许对CEA进行更早，更方便的测试，并最终用于其他肿瘤标记。