Upconversion nanoparticle-based optical biosensor for early diagnosis of stroke

基于上转换纳米粒子的光学生物传感器用于中风的早期诊断

• 批准号: MR/Y503460/1
• 负责人: Sikha Saha
• 金额: $ 31.27万
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FY503460%2F1
• 关键词: Upconversion; nanoparticle; based; optical; biosensor

Stroke is currently diagnosed by physical tests and studying images of brain scans to determine if the stroke has been caused by ischaemia (when a blockage cuts off the blood supply to the brain, e.g., blood clot) or a haemorrhage (blood vessel in the brain ruptures and causes bleeding inside the brain). Brain scans can reliably confirm bleeding, but many patients with suspected ischaemic stroke (up to 40%) are subsequently confirmed to have alternative pathologies e.g., migraine. seizures (stroke mimics) delaying transfer of stroke patients to acute stroke unit for early intervention and treatment. Thus, there is an urgent need for a biomarker for brain attack (stroke) just like there is one for heart attack. The current 'Gold Standard' technique for the detection of protein biomarkers is complex, time-consuming and requires automated equipment. Thus, it is important to develop and validate a rapid diagnostic tool for detection of blood biomarkers for stroke in the ambulance and Emergency Department, where a significant clinical problem lies i.e., the need to make a fastaccurate diagnosis of stroke. A simple and complimentary point of care (POC)biomarker test to differentiate stroke patients from non-stroke patients with similar clinical symptoms is essential in prehospital and emergency settings for efficient stroke management and early treatment. We have now developed a proof of principle biosensing tool for rapid analysis of potential biomarker in human blood serum. The proposed project focuses on the development and validation of a POC tool for early diagnosis of stroke-specific biomarkers to rapidly identify stroke patients. The POC biomarker assessment could be performed during initial triage avoiding delays in transporting stroke patients to appropriate care centres allowing expedited treatment to risk patients for better survival with improved quality of life. The proposed stroke of non stroke patients thus improving stroke patient flow and reducing expenses to the health care organisation.

目前，中风的诊断是通过物理测试和研究脑部扫描图像来确定中风是否是由缺血（当阻塞切断大脑的血液供应，例如血栓）或出血（大脑中的血管）引起的。破裂并导致脑内出血）。脑部扫描可以可靠地确认出血，但许多疑似缺血性中风的患者（高达 40%）随后被证实患有其他病症，例如偏头痛。癫痫发作（类似中风）延迟将中风患者转移到急性中风病房进行早期干预和治疗。因此，迫切需要一种脑部发作（中风）的生物标志物，就像心脏病发作的生物标志物一样。目前检测蛋白质生物标志物的“黄金标准”技术复杂、耗时，并且需要自动化设备。因此，开发和验证一种快速诊断工具来检测救护车和急诊室中风的血液生物标志物非常重要，其中一个重要的临床问题在于，需要对中风进行快速准确的诊断。在院前和急诊环境中，进行简单且免费的护理点 (POC) 生物标志物测试来区分中风患者与具有相似临床症状的非中风患者对于有效的中风管理和早期治疗至关重要。我们现已开发出一种原理证明生物传感工具，用于快速分析人血清中的潜在生物标志物。拟议项目的重点是开发和验证用于早期诊断中风特异性生物标志物的 POC 工具，以快速识别中风患者。 POC 生物标志物评估可以在初始分诊期间进行，避免延误将中风患者运送到适当的护理中心，从而加快治疗风险，从而提高患者的生存率并提高生活质量。建议非中风患者中风，从而改善中风患者流量并减少医疗保健机构的费用。