XAS OF BIOINORGANIC SYSTEMS

生物无机系统的 XAS

• 批准号: 7370620
• 负责人: PETER A LAY
• 金额: $ 0.41万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370620
• 关键词: XAS; BIOINORGANIC; SYSTEMS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Changes in the heme environment when the a-hemoglobin stabilizing protein (AHSP) binds to a-hemoglobin (a-Hb), which we have studied by Raman spectroscopy, will be monitored by Fe K-edge XAFS. Such binding is important in prevention of toxic heme precipitation from unstable oxy-a-Hb, but all crystal structures of AHSP:oxy-a-Hb complexes contain mutant Hb and it is not clear whether the structures are the same as with the wild type because they have different reactivities. Fe K-edge XAFS will also be used to study the reactions of myoglobin with H2O2 in cardiac myocytes. Such processes are thought to lead ultimately to cardiovascular disease and XAS will be recorded on snap frozen cell pellets of the myocytes (using previously developed techniques) that are untreated or H2O2-treated and changes in the Mb XAS will be monitored. Cr K-edge XAFS will be recorded on Cr(VI) adducts of phosphatase enzymes (for which there are no structural data), since we have recently shown that anti-diabetic effects of commonly used Cr(III) dietary supplements are likely to be exerted by in-vivo enzymatic oxidation of the Cr(III) dietary supplements to carcinogenic Cr(VI), followed increased insulin activity due to potent Cr(VI) inhibition of phosphatase enzymes We also intend to study the very early stages of the biotransformations of Cr(VI) in human lung cells to complement our recent work in the area.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。当A-血红蛋白稳定蛋白（AHSP）与A-Hemoglobin（A-HB）结合时，血红素环境的变化将通过Raman Spectroscopicy研究的A-血红蛋白（A-HB），由Fe K-gede XAFS监测。这种结合对于预防不稳定的Oxy-A-Hb的毒性血红素沉淀很重要，但是AHSP的所有晶体结构：Oxy-A-Hb复合物都含有突变的Hb，尚不清楚结构是否与野生型相同，因为它们具有不同的反感。 Fe K-Edge XAFS还将用于研究肌红蛋白与H2O2在心肌细胞中的反应。人们认为这种过程最终导致心血管疾病，XAS将记录在肌细胞的快照冷冻细胞颗粒上（使用先前开发的技术），这些细胞未经处理或接受H2O2处理，并将监测MB XAS的变化。 Cr k-edge XAF将记录在磷酸酶的CR（VI）加合物上（对于没有结构数据），因为我们最近表明，常用CR（III）饮食补充剂的抗糖尿病作用可能会因CR（III）饮食的饮食（III）饮食（III）饮食（III）的饮食（III）的含量（III）的含量（III IIN）的含量（III）的含量（III IIN）施加（III IIN）施加（III IIN）。 CR（VI）抑制磷酸酶酶，我们还打算研究人类肺细胞中Cr（VI）生物转化的早期阶段，以补充我们最近在该地区的工作。