INTRACELLULAR XAS ON CULTURED MAMMALIAN CELLS TREATED WITH CR CARCINOGENS & DIET

用 CR 致癌剂处理的培养哺乳动物细胞的细胞内 XAS

• 批准号: 7598011
• 负责人: PETER A LAY
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7598011
• 关键词: Adverse effects; Animals; Carcinogen Metabolism; Carcinogens; Cell Line; Cells; Chemistry; Complex; Computer Retrieval of Information on Scientific Projects Database; Cultured Cells; Diet; Electron Transport Complex III; Environment; Environmental Carcinogens; Funding; Grant; Human; Image; Institution; Ligands; Lung; Malignant Neoplasms; Malignant neoplasm of lung; Mammalian Cell; Measurement; Metabolic Biotransformation; Metalloproteins; Metals; Modeling; Muscle Cells; Mutagens; Occupational; Permeability; Pharmaceutical Preparations; Research; Research Personnel; Resources; Sampling; Source; Structure; Techniques; Tissues; United States National Institutes of Health; chromium hexavalent ion; dietary supplements; interest; metabolic abnormality assessment; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The measurement of intracellular XANES and XAFS of bulk samples of cultured cells or of tissues can be used as a complementary technique to micro-XANES to determine the structures of metalloproteins, drugs, and metal carcinogens in an intracellular environment. Studies on Cr(VI) are of considerable interest as it is a widespread occupational and environmental carcinogen and, similarly, Cr(III) complexes are of interest because they have widespread applications as human and animal dietary supplements. In the current program, the cellular permeability and intracellular biotransformations of Cr carcinogens, mutagens, and dietary supplements will be studied in relation to Cr-induced cancers and the efficacy and side-effects of Cr dietary supplements. Specifically, the metabolism of carcinogens and mutagens will be studied in mammalian lung cells (the target for Cr-induced lung cancers). The intracellular chemistry of the dietary supplements will be studied with the same lung cell lines (to assess potential carcinogenic side-effects) and with human muscle cells to study the metabolism of these supplements and the effects on target cells. XANES imaging will also be examined for the differentiated muscle cells, which can be larger than 100 ¿m long. The XANES and XAFS will be compared with those of model complexes of Cr with biologically relevant ligands. Where there is evidence that the XANES is dominated by only one or two complexes, XAFS will also be used in order to investigate structural information in more detail.

该副本是使用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这是调查员的机构。 在细胞内环境中，测量培养细胞或组织的细胞内XANE和XAFS可以用作微Xanes的完整技术，以确定金属蛋白，药物和金属致癌物的结构。关于CR（VI）的研究具有考虑，因为它是一种宽度占据的占用和环境致癌物，并且同样，CR（III）复合物引起了人们的关注，因为它们具有人类和动物饮食补充剂的宽度应用。在当前的程序中，将研究有关CR诱导的癌症以及CR饮食补充剂的有效性和副作用，将研究CR致癌物，诱变剂和饮食补充剂的细胞渗透性和细胞内生物转化。具体而言，将在哺乳动物肺细胞（CR诱导的肺癌的靶标）中研究致癌物和诱变剂的代谢。将使用相同的肺细胞系（评估潜在的致癌副作用）和人类肌肉细胞研究饮食补充剂的细胞内化学，以研究这些补充剂的代谢以及对靶细胞的影响。还将检查XANES成像的分化肌肉细胞，该肌肉细胞可能大于100`m。 XANE和XAF将与具有生物学相关配体的CR模型复合物的XAF进行比较。如果有证据表明Xanes仅由一个或两个复合物主导，则还将使用XAFS来更详细地研究结构信息。