TRAF MOLECULES IN CELL SIGNALING

细胞信号转导中的 TRAF 分子

• 批准号: 7370479
• 负责人: KATHRYN R. ELY
• 金额: $ 0.02万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7370479
• 关键词: TRAF; MOLECULES; CELL; SIGNALING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Tumor necrosis factor receptors are cytokine receptors important in cellular signaling. CD40 is a well-characterized member of this family that is expressed on B cells and triggers immune responses, immunoglobulin class switching and activation of apoptosis. Like CD40, other TNFRs, such as LTbeta receptor and BAFF receptor, as well as the oncoprotein LMP1 from Epstein Barr Virus, associate with the cytoplasmic domain of TRAFs to stimulate cell signals. In a continuing series, we are crystallizing TRAF3 bound in complex with the functional fragments of each of these signaling partners. To study the details of binding and define the molecular basis for signaling, synthetic peptides representing the interacting regions are soaked into TRAF3 crystals. The crystals do not typically diffract with a standard rotating anode source to the level of resolution required for detailed binding analyses. Therefore we plan to continue using synchrotron x-rays to collect data for the complexes.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。肿瘤坏死因子受体是细胞因子受体在细胞信号中重要的受体。 CD40是该家族中特征良好的成员，在B细胞上表达，并触发免疫反应，免疫球蛋白类切换和凋亡的激活。与CD40一样，其他TNFR，例如LTBETA受体和BAFF受体，以及来自Epstein Barr病毒的Oncoprotein LMP1，与TRAFS的细胞质结构域相关，以刺激细胞信号。在一个持续的序列中，我们正在结晶TRAF3与每个信号伴侣的功能片段结合在一起。为了研究结合的细节并定义了信号传导的分子基础，将代表相互作用区域的合成肽浸泡在TRAF3晶体中。晶体通常不会将标准旋转阳极源衍射到详细结合分析所需的分辨率水平。因此，我们计划继续使用Synchrotron X射线来收集复合物的数据。