Complex Nanocomposites for Bone Regeneration

用于骨再生的复杂纳米复合材料

• 批准号: 7067560
• 负责人: ANTONI P TOMSIA
• 金额: $ 68.08万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-04 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7067560
• 关键词: 3T3 cells; biomaterial compatibility; biomaterial development /preparation; biomaterial evaluation; biomechanics; biomimetics; bone regeneration; calcium phosphate; cell adhesion molecules; gel; human tissue; hydroxyapatites; immunocytochemistry; laboratory rat; mechanical stress; medical implant science; nanotechnology; particle; polymers; scanning electron microscopy; tissue /cell culture; tissue support frame

DESCRIPTION (provided by applicant): This Bioengineering Research Partnership proposal is submitted by a multidisciplinary collaboration of scientists primarily affiliated with the University of California (UC) system. The lead institution is Lawrence Berkeley National Laboratory, with component groups at UC Berkeley and San Francisco campuses. There is also small business collaborator from SkeleTech, Inc., in Bothell, WA. Some of the collaborators have worked together on ceramic projects for over 20 years, while others have worked together on dental research projects for over 10 years. This team has been expanded to include greater expertise in all the disciplines involved in this proposal: materials science, chemistry, biology, and dental/medical science. The research is aimed at development and testing of new implant materials by combining biomimetics with two radically new design philosophies to produce dense and strong bioactive scaffolds that are intended to be partially or completely resorbed and replaced by bone from the host in a sequence resembling bone remodeling. The ultimate goal is to develop strong and tough implant materials for load-bearing applications deriving their strength from nanoparticle hydroxyapatite and their toughness from hydrogel polymers, with the microstructural architecture scale on the order of tens of nanometers and below. Three types of materials will be developed. First, inorganic scaffolds with a dense core and a graded distribution of porosity and surface chemistry will be fabricated by stereolithography and by a novel technology developed in our laboratory based on freeze casting of calcium phosphate suspensions. Second, hydrogels and self-assembling polymers that possess anionic groups and adhesive ligands suitably positioned for the nucleation process and cellular adhesion will be used to direct templatedriven biomimetic mineralization of hydroxyapatite and other biominerals in nanoscopically and microscopically controlled fashion. Third, the resultant porous scaffolds will be used as the matrices to fabricate inorganic-organic composites with improved strength and fracture resistance. This will be achieved by infiltration of the inorganic scaffolds with hydrogels or by direct template-driven biomimetic mineralization of calcium phosphate nanoparticles on the organic scaffolds. Materials that pass the mechanical property tests will be tested in cell cultures and an animal model. Improvement of implants will result in improved health and quality of life for the millions of people who will need implants in the future.

描述（由申请人提供）： 该生物工程研究合作伙伴提案由主要隶属于加州大学 (UC) 系统的多学科科学家合作提交。牵头机构是劳伦斯伯克利国家实验室，在加州大学伯克利分校和旧金山校区设有组成小组。还有来自华盛顿州博塞尔的 SkeleTech, Inc. 的小型企业合作者。一些合作者在陶瓷项目上合作了 20 多年，而另一些合作者在牙科研究项目上合作了 10 多年。该团队已扩大到包含该提案涉及的所有学科的更丰富的专业知识：材料科学、化学、生物学和牙科/医学。该研究旨在开发和测试新的植入材料，将仿生学与两种全新的设计理念相结合，生产致密且坚固的生物活性支架，这些支架旨在部分或完全被宿主的骨骼吸收和取代，顺序类似于骨重塑。最终目标是开发用于承载应用的坚固而坚韧的植入材料，其强度来自纳米颗粒羟基磷灰石，韧性来自水凝胶聚合物，其微观结构规模约为数十纳米及以下。将开发三种类型的材料。首先，将通过立体光刻和我们实验室开发的基于磷酸钙悬浮液冷冻铸造的新技术来制造具有致密核心和孔隙率和表面化学梯度分布的无机支架。其次，具有阴离子基团和适合成核过程和细胞粘附的粘附配体的水凝胶和自组装聚合物将用于以纳米级和微观控制的方式指导羟基磷灰石和其他生物矿物的模板驱动仿生矿化。第三，所得的多孔支架将用作基体来制造具有改进的强度和抗断裂性的无机-有机复合材料。这将通过水凝胶渗透无机支架或通过有机支架上磷酸钙纳米颗粒的直接模板驱动仿生矿化来实现。通过机械性能测试的材料将在细胞培养物和动物模型中进行测试。植入物的改进将改善未来数百万需要植入物的人的健康和生活质量。