Small Animal Laboratory Core

小动物实验室核心

• 批准号: 7028461
• 负责人: BRIAN A. HERMAN
• 金额: $ 21.5万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2010-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7028461
• 关键词: biomedical facility; bone disorder; cell line; computed axial tomography; disease /disorder model; health science research support; host neoplasm interaction; laboratory mouse; molecular /cellular imaging; molecular oncology; morphometry; neoplastic cell; neoplastic growth; positron emission tomography; single photon emission computed tomography; statistics /biometry; tissue /cell preparation

The theme of this Program Project Grant is heavily based around the use of preclinical in vivo models to study the molecular mechanisms involved with the effects to cancer on the skeleton. Each project requires the use of animal models and careful assessment and monitoring of those animals for both skeletal and soft tissue metastases. The Small Animal Core proposed here will provide support for all work involving animals within each individual project. Services will include maintenance of tumor cell lines, management of all procedures involving small animals as well as state-of-the art methods for assessing the effects of cancer on the skeleton. This core builds on the previously existing laboratory core by incorporating new techniques such as optical imaging of green fluorescent protein expressing tumor cells, MicroSPECT, MicroPET and microCT for the assessment of tumor effects of the skeleton and tumor burden. An additional strength of the Small Animal Core is the participation of recognized experts in these areas. Techniques such as MicroSPECT, Micro PET and MicroCT are used in clinical diagnostics for patients with both breast cancer and multiple myeloma but to our knowledge have not yet been utilized in small animal models of metastatic disease. Use of these techniques will allow us to make direct comparisons between our preclinical models and disease in patients providing for validation of our preclinical models of breast cancer and multiple myeloma. Furthermore, these techniques will build on those that we have used in the past such as radiography and histomorphometry by allowing us to obtain a much more thorough picture of tumor burden and distribution in the whole intact animal, while providing much better resolution.

该计划项目拨款的主题很大程度上基于临床前体内的使用 研究癌症对骨骼影响的分子机制的模型。 每个项目都需要使用动物模型并对这些模型进行仔细评估和监测 动物骨骼和软组织转移。这里提出的小动物核心将 为每个项目中涉及动物的所有工作提供支持。服务将包括 肿瘤细胞系的维护，涉及小动物的所有程序的管理以及 评估癌症对骨骼影响的最先进方法。该核心建立在 通过结合光学成像等新技术，以前现有的实验室核心 表达绿色荧光蛋白的肿瘤细胞、MicroSPECT、MicroPET 和 microCT 评估肿瘤对骨骼的影响和肿瘤负荷。小号的额外优势 Animal Core 是这些领域公认专家的参与。技术如 MicroSPECT、Micro PET 和 MicroCT 用于双侧乳房患者的临床诊断 癌症和多发性骨髓瘤，但据我们所知尚未在小动物中使用 转移性疾病模型。使用这些技术将使我们能够进行直接比较 我们的临床前模型和患者疾病之间的关系为我们的临床前模型提供了验证 乳腺癌和多发性骨髓瘤模型。此外，这些技术将建立在那些 我们过去使用过的方法，例如放射线照相术和组织形态测量法，使我们能够获得 更全面地了解整个完整动物中的肿瘤负荷和分布，同时 提供更好的分辨率。