MOUSE PHENOTYPING, PHYSIOLOGY, AND METABOLISM CORE

小鼠表型、生理学和代谢核心

基本信息

批准号：
7284633
负责人：
REXFORD S. AHIMA
金额：
$ 17.3万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7284633
关键词：
Arts Biological Markers Body Composition Bone Tissue Cellular biology Chemicals Chemistry Consult Consultations Coupled Data Data Collection Databases Diabetes Mellitus Diabetic mouse Diagnostic Diagnostic Services Diagnostic tests Diet Dose Employment Endocrinology Energy Metabolism Ensure Environmental Risk Factor Equipment Exercise Experimental Designs Failure Fatty acid glycerol esters Fee-for-Service Plans Funding Funding Agency Future Gene Targeting Genomics Glucose Home environment Hormonal Housing Human Human Resources Injection of therapeutic agent Insulin Insulin Resistance Intravenous Islet Cell Islets of Langerhans Kinetics Knockout Mice Laboratories Laboratory Animals Laboratory mice Lipids Magnetic Resonance Magnetic Resonance Imaging Measurement Measures Metabolic Metabolism Methodology Mission Molecular Monitor Motor Activity Mus Muscle Mutant Strains Mice Neonatal Obese Mice Obesity Operative Surgical Procedures Oral Oxygen Consumption Pathogenesis Pharmacological Treatment Phenotype Physiological Physiology Procedures Radioactive Tracers Radioimmunoassay Research Research Personnel Rest Screening procedure Services Specialist System Temperature Testing Tissues Transgenic Organisms Translations United States National Institutes of Health Water Week Wolves blood glucose regulation cost data acquisition day design drinking feeding glucose uptake in vivo instrument insulin sensitivity insulin tolerance intraperitoneal member mouse model research study respiratory

项目摘要

MOUSE PHENOTYPING, PHYSIOLOGY, AND METABOLISM CORE: Director - R. Ahima Our understanding of the pathogenesis of diabetes has benefited from the use of gene targeting methodology in mice to elucidate molecular mechanisms. However, such efforts are often hampered by an absence of a clear metabolic phenotype. Failure to identify a phenotype may be due to lack of expertise and/or facilities for evaluating metabolic changes in mice. The Mouse Phenotyping, Physiology and Metabolism Core provides investigators of the Penn Diabetes and Endocrinology Research Center (DERC) with state-of-the-art, timely and cost-effective diagnostic studies in mice. The core offers consultation and experimental design, monitoring of feeding, energy expenditure and locomotor activity using the Comprehensive Laboratory Animal Monitoring System (CLAMS), treadmill exercise using the Oxymax system, and measurement of body composition using dual emission x-ray absorptiometry (DEXA) and carcass chemistry. Glucose homeostasis is assessed by oral or intraperitoneal (i.p.) glucose administration, and whole body insulin sensitivity by i.p. insulin injection. Insulin clamp and radioactive tracers are used to assess glucose fluxes and tissue specific glucose uptake. Studies in the core are performed by two research specialists under the direction of Rex Ahima. Future plans for the core include the use magnetic resonance (MRI) for measurement of water, lean and fat content, assessment of in vivo lipid kinetics, and employment of an additional technician to expedite services. The Mouse Phenotyping, Physiology and Metabolism Core will maintain a databank of metabolic and hormonal parameters in mouse models of diabetes and obesity, and coordinate its activities with other core laboratories, i.e. Islet Cell Biology (Franz Matschinsky), Radioimmunoassay/Biomarkers (Bryan Wolf; Muredach Reilly), Transgenic and Chimeric Mouse (Nancy Cooke), and Genomics and Gene Targeting Cores (Klaus Kaestner). These efforts will result in optimum data acquisition and metabolic phenotyping of mice, and facilitate the translation of ideas from the bench to mice, and ultimately to humans.

鼠标表型，生理和代谢核心：导演-R。Ahima 我们对糖尿病发病机理的理解已受益于使用基因靶向小鼠阐明分子机制的方法。但是，这种努力经常是由于没有明确的代谢表型而阻碍了。无法识别表型可能是由于缺乏评估小鼠代谢变化的专业知识和/或设施。鼠标表型，生理和代谢核心为宾夕法尼亚州糖尿病和内分泌研究中心（DERC），及时，及时且具有成本效益的诊断在小鼠中的研究。核心提供咨询和实验设计，监视喂养，使用综合实验室动物监测的能量消耗和运动活动系统（蛤），使用Oxymax系统进行跑步机运动以及身体的测量使用双发射X射线吸收法（DEXA）和car体化学的组成。葡萄糖稳态由口服或腹膜内（i.p.）葡萄糖给药和全身评估 I.P.胰岛素敏感性胰岛素注射。胰岛素夹和放射性示踪剂用于评估葡萄糖通量和组织特异性葡萄糖摄取。核心的研究由两个在雷克斯·阿米马（Rex Ahima）的指导下，研究专家。核心的未来计划包括使用磁共振（MRI）用于测量水，瘦和脂肪含量，体内评估脂质动力学以及雇用额外的技术人员来加速服务。鼠标表型，生理和代谢核心将保持代谢和激素的数据库糖尿病和肥胖的小鼠模型中的参数，并与其他核心协调其活动实验室，即胰岛细胞生物学（Franz Matschinsky），放射免疫测定/生物标志物（Bryan 狼; MUREDACH Reilly），转基因和嵌合小鼠（Nancy Cooke）以及基因组学和基因靶向核心（Klaus Kaestner）。这些努力将导致最佳数据获取和小鼠的代谢表型，并促进思想从板凳向小鼠的翻译，以及最终对人类。