ABI 3730XL DNA ANALYZER: BREAST CANCER, AGING AND CANCER, CARCINOMA

ABI 3730XL DNA 分析仪：乳腺癌、衰老和癌症、癌症

• 批准号: 7335250
• 负责人: DAVID Franklin BISHOP
• 金额: $ 5.61万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-15 至 2007-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335250
• 关键词: DNA; breast neoplasms; carcinoma; gene mutation; genes; genotype; neoplasm /cancer; nucleic acid sequence

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The overall objective of this NCRR Shared Instrument Grant application is to upgrade and update a heavily-used Institutional Shared DNA Sequencing and Genotyping Core (DNAcore) with an ABI 3730xl DNA Analyzer to 1) lower costs of DNA sequencing and genotyping, 2) increase sequencing read-length and quality, and to 3) provide the new high-throughput ABI SNPlex method for SNP analysis. The requested ABI 3730xl DNA Analyzer is the current state-of-the-art capillary array analyzer that will fulfill all these goals and provide the economy and flexibility needed to satisfy the multitude of user projects requiring our shared resource facility. Of great importance to our investigators working on gene association projects, it will support low to high throughput genotyping utilizing a wide variety of approaches including STR-based linkage, single SNP analyses, allele quantitation by SNaPshot assays, and high throughput genotyping with 48-plex SNPlex assays. This upgrade to our busy shared sequencing, genotyping and oligonucleotide synthesis core facility at Mount Sinai will replace the ageing ABI 3700 platform that is too expensive to run due to its much greater use of reagents, its downtime due to hardware failures, its inability to support the SNPlex assay and its reduced signal sensitivity and uniformity relative to the new ABI 3730xl. This application is strengthened by the previous 10 years of experience of its Principal Investigator in providing high-quality DNA sequencing, genotyping, mutation detection and oligonucleotide synthesis for rapidly increasing numbers of NIH and other investigators at Mount Sinai. The DNAcore provides in-house expertise and consultation services for all sequencing, genotyping, positional cloning, mutation detection, and LOH projects. Equitable, cost-effective and responsive utilization of the requested instrumentation will be promoted through the DNAcore's Local Advisory Committee. They will also identify and recommend novel strategies, technologies, instrumentation, and resources to further facilitate and foster cutting-edge research. The Dean for Research will support service contracts and provide $100K towards the purchase of the requested ABI 3730xl, which with the $65K trade-in for the old ABI 3700 will make this request more cost effective. In addition, the Dean will support technician salaries after another grant terminates next year. With the requested ABI 3730xl DNA Analyzer, the DNAcore will continue to meet intense needs of our over 200 investigators with over 250 NIH grants for economical and accessible on-site sequencing and genotyping services to speed gene discovery, gene structure analysis, mutation characterization, analysis of vector constructs, linkage analysis, genome scanning, LOH studies, etc. In summary, the requested equipment upgrade will fit synergistically with existing resources and our Dean's major genomics initiatives to enhance research productivity by reducing costs of these key services to investigator's grant funds and providing higher quality sequencing and hitherto unavailable analyses to support high-throughput genomic scans.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：此 NCRR 共享仪器拨款申请的总体目标是使用 ABI 3730xl DNA 分析仪升级和更新频繁使用的机构共享 DNA 测序和基因分型核心 (DNAcore)，以 1) 降低 DNA 测序成本和基因分型，2) 增加测序读长和质量，3) 为 SNP 分析提供新的高通量 ABI SNPlex 方法。所需的 ABI 3730xl DNA 分析仪是当前最先进的毛细管阵列分析仪，它将实现所有这些目标，并提供满足需要我们共享资源设施的众多用户项目所需的经济性和灵活性。对于我们从事基因关联项目的研究人员来说非常重要，它将支持利用多种方法进行低到高通量基因分型，包括基于 STR 的连锁、单一 SNP 分析、通过 SNaPshot 测定进行等位基因定量以及使用 48 重进行高通量基因分型SNPlex 检测。对我们位于西奈山繁忙的共享测序、基因分型和寡核苷酸合成核心设施的升级将取代老化的 ABI 3700 平台，该平台由于试剂使用量大、硬件故障导致的停机以及无法支持而运行成本过高。与新的 ABI 3730xl 相比，SNPlex 检测的信号灵敏度和均匀性有所降低。该应用程序因其首席研究员过去 10 年在为数量迅速增加的 NIH 和西奈山其他研究人员提供高质量 DNA 测序、基因分型、突变检测和寡核苷酸合成方面的经验而得到加强。 DNAcore 为所有测序、基因分型、定位克隆、突变检测和 LOH 项目提供内部专业知识和咨询服务。 DNAcore 的地方咨询委员会将促进对所需仪器的公平、具有成本效益和快速响应的利用。他们还将确定并推荐新颖的战略、技术、仪器和资源，以进一步促进和培育尖端研究。研究院长将支持服务合同并提供 10 万美元用于购买所请求的 ABI 3730xl，加上旧 ABI 3700 的 6.5 万美元以旧换新将使该请求更具成本效益。此外，院长将在明年另一笔拨款终止后支持技术人员的工资。借助所需的 ABI 3730xl DNA 分析仪，DNAcore 将继续满足我们 200 多名研究人员的强烈需求，并获得 250 多项 NIH 拨款，提供经济且易于使用的现场测序和基因分型服务，以加速基因发现、基因结构分析、突变表征、分析载体构建、连锁分析、基因组扫描、LOH 研究等。总之，所要求的设备升级将与现有资源和我们院长的主要基因组学计划协同配合通过降低研究人员资助资金的这些关键服务的成本并提供更高质量的测序和迄今为止无法实现的分析来支持高通量基因组扫描，从而提高研究生产力。