Array of Micromachined UltraSonic Electrosprays Bioanalytical Mass Spectrometry

微机械超声电喷雾生物分析质谱阵列

• 批准号: 7286862
• 负责人: ANDREI G FEDOROV
• 金额: $ 17.28万
• 依托单位: GEORGIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7286862
• 关键词: Biological; Biological Markers; Caliber; Charge; Cyclotrons; Detection; Development; Device or Instrument Development; Devices; Electrospray Ionization; Human; Ions; Mass Spectrum Analysis; Measures; Molecular Weight; Monitor; Operative Surgical Procedures; Peptides; Performance; Phase; Process; Property; Protein Analysis; Proteins; Proteomics; Public Health; Range; Rate; Relative (related person); Reproducibility; Sample Size; Sampling; Scientist; Silicon; Solvents; Source; Standards of Weights and Measures; Stream; Technology; Testing; Time; Ultrasonics; Variant; base; cancer type; concept; design; drug development; improved; ion source; mass spectrometer; nano-electrospray; novel; prototype; response; success; voltage

DESCRIPTION (provided by applicant): We propose to develop a novel micromachined ultrasonic electrospray source which eliminates most, if not all, limitations of the conventional ESI technology, thereby providing scientists involved in biomedicine, functional proteomics and biomarker discovery with a unique MS interface for high throughput, ultrasensitive, and multiplexed analysis of proteins mixtures of biological significance. The proposed technology called AMUSE (Array of Micromachined UltraSonic Electrospray) ion source has potential for operation at low voltages with wide range of solvents, capable of minimizing the required sample size and improving sample utilization, and is inherently suitable for parallel, high throughput operation with multiplexing in the array format. Further, the ultrasonic electrospray source can be made inexpensive to be disposable since it is batch microfabricated using a simple process. We will design and fabricate the prototype(s) of the micromachined ultrasonic electrospray source array for use in mass spectrometric analysis of protein mixtures of biological relevance. We will test and optimize the performance of both ESI device concepts and to clearly quantify their potential advantages. We will evaluate the analytical performance of a time-of-flight mass spectrometer outfitted with the proposed micromachined ultrasonic electrospray source and compare it with existing commercial nanospray ion sources. Development of an improved electrospray source for protein mass spectrometry, as proposed here, would enable identification of new biomarkers of various human deceases, including various types of cancers. In addition, mass spectrometry is routinely used in drug development, and therefore the advancement of electrospray mass spectrometry will contribute directly to improvement in public health.

描述（由申请人提供）： 我们建议开发一种新型微机械超声电喷雾源，消除传统 ESI 技术的大部分（如果不是全部）限制，从而为参与生物医学、功能蛋白质组学和生物标志物发现的科学家提供独特的 MS 接口，实现高通量、超灵敏和多重分析。具有生物学意义的蛋白质混合物的分析。所提出的技术称为 AMUSE（微机械超声电喷雾阵列）离子源，具有在低电压下使用多种溶剂运行的潜力，能够最大限度地减少所需的样品尺寸并提高样品利用率，并且本质上适合并行、高通量操作以数组格式进行复用。此外，由于超声电喷射源是使用简单的工艺批量微制造的，因此超声电喷射源可以廉价地制成一次性的。我们将设计和制造微机械超声电喷雾源阵列的原型，用于生物相关蛋白质混合物的质谱分析。我们将测试和优化这两种 ESI 设备概念的性能，并明确量化它们的潜在优势。我们将评估配备所提出的微机械超声电喷雾源的飞行时间质谱仪的分析性能，并将其与现有的商业纳喷雾离子源进行比较。正如本文所提出的，开发用于蛋白质质谱分析的改进电喷雾源将能够识别各种人类疾病（包括各种类型的癌症）的新生物标志物。此外，质谱法常用于药物开发，因此电喷雾质谱法的进步将直接有助于改善公众健康。