Structure and Function of Human Salivary Mucins

人唾液粘蛋白的结构和功能

基本信息

批准号：
7424055
负责人：
GWYNNETH D OFFNER
金额：
$ 32.27万
依托单位：
BOSTON MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-08-01 至 2010-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7424055
关键词：
Affect Amylases Anabolism Antibodies Binding Biological Biological Assay Biological Models Biological Process COS-7 Cell Cell Line Cell model Cloning Complex Condition Deglutition Desiccation Diffusion Dimerization Environment Epithelial Exhibits Gel Gel Chromatography Genes Glycoproteins Goals Host Defense Human Human Genome Project Hybrids Immunohistochemistry In Situ Hybridization Lactoferrin MUC5B gene Mammalian Cell Mass Spectrum Analysis Mastication Measures Minor salivary gland structure Molecular Molecular Weight Muc5B protein Mucins N-terminal Names Nature Oral Oral cavity Pathway interactions Pattern Peptides Polymerase Chain Reaction Precipitation Process Property Proteins Proteomics Relative (related person)Research Personnel Rest Role Saliva Salivary Salivary Proteins Silicon Dioxide Speech Structure Sublingual Gland Surface Thinking Time Variant Weight antimicrobial base dimer gel electrophoresis histidine-rich proteins immunoaffinity chromatography monomer oral bacteria programs proline-rich proteins reconstitution saliva secretion salivary mucins size soft tissue statherin yeast two hybrid system

项目摘要

DESCRIPTION: Two distinct mucins occur in the secretions of submandibular, sublingual and minor salivary glands, a large multimeric gel forming mucin named MG1 and a small monomeric mucin named MG2. MG1 appears to be a mixture of two mucin gene products, MUC5B and a newly identified mucin, MUC19. MG2 appears to contain a single mucin gene product, MUC7. Mucins protect oral surfaces against desiccation, lubricate hard and soft tissues to facilitate mastication, speech and swallowing and form a selectively permeable diffusion barrier between underlying epithelial surfaces and the external environment. We have shown that both MG1 and MG2 form heterotypic complexes with other salivary proteins and posit that many of the functions previously attributed to mucins can be ascribed to the nature of their complex partners. The overall goal of this project is to understand how the molecular composition of mucins and mucin complexes in salivary secretions and whole saliva affects their biological function in the oral cavity. The Specific Aims of this project are to: 1) Identify salivary proteins that form complexes with MG1 using a mass spectrometry based proteomics approach; 2) Isolate and identify salivary proteins that interact with MG2; 3) Explore the functional properties of mucin complexes using assays to measure the specific biological properties of complex partners; 4) Investigate the mechanism for assembly of gel-forming mucins expressed in mammalian cell lines; and 5) Determine the molecular composition and secretion patterns of gel forming mucins in whole saliva and salivary secretions using capture ELISAs.

描述：下颌，舌下和次要唾液腺的分泌物中出现了两种不同的粘蛋白，一种称为MG1的大型多聚体凝胶形成粘蛋白和一个名为MG2的小型单体粘蛋白。 MG1似乎是两种粘蛋白基因产物MUC5B和新鉴定的粘蛋白MUC19的混合物。 MG2似乎包含单个粘蛋白基因产物MUC7。粘蛋白可保护口腔表面免受干燥，润滑硬组织和软组织，以促进咀嚼，言语和吞咽，并在潜在的上皮表面和外部环境之间形成可有选择性的渗透性扩散屏障。我们已经表明，MG1和MG2都与其他唾液蛋白形成异型复合物，并认为以前归因于粘蛋白的许多功能都可以归因于其复杂伴侣的性质。该项目的总体目标是了解唾液分泌物中粘蛋白和粘蛋白复合物的分子组成如何影响其在口腔中的生物学功能。该项目的具体目的是： 1）使用基于质谱的蛋白质组学方法来确定与MG1形成复合物的唾液蛋白； 2）分离并鉴定与MG2相互作用的唾液蛋白； 3）使用测定法探索粘蛋白复合物的功能特性，以测量复杂伴侣的特定生物学特性； 4）研究在哺乳动物细胞系中表达的凝胶形成粘蛋白组装的机制；和 5）使用捕获ELISAS确定整个唾液和唾液分泌中凝胶形成粘蛋白的分子组成和分泌模式。