Membrane Bound Mucins in Salivary Glands and Saliva

唾液腺和唾液中的膜结合粘蛋白

• 批准号: 6611418
• 负责人: GWYNNETH D OFFNER
• 金额: $ 24.15万
• 依托单位: BOSTON MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2005-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6611418
• 关键词: bacteria infection mechanism; cell line; clinical research; enzyme linked immunosorbent assay; epithelium; gene expression; host organism interaction; human tissue; immunocytochemistry; membrane proteins; mucins; oral bacteria; oral mucosa; protein localization; protein protein interaction; saliva; salivary glands; western blottings; yeast two hybrid system

DESCRIPTION (provided by applicant): Mucins are heavily glycosylated proteins, which are synthesized and secreted by epithelial cells lining the gastrointestinal, respiratory and genitourinary tracts. To date, thirteen distinct mucin genes have been identified and these can be divided into two distinct classes, gel-forming mucins and membrane-bound mucins. In salivary secretions, mucins facilitate speech and swallowing, protect oral surfaces against microbial, chemical and mechanical injury and contribute to a selectively permeable diffusion barrier between underlying surfaces and the external environment. These functions have largely been attributed to the major gel-forming mucin in saliva, MG1. Nevertheless, we have recently shown that two membrane-bound mucins, MUC1 and MUC4, are expressed in all major salivary glands, including the parotid glands. While virtually nothing is known about the expression and functional properties of membrane-bound mucins in salivary glands and saliva, our hypothesis is that these molecules form an integral part of the oral host-defense system in the protection of oral epithelial surfaces. The overall goal of this project is to understand the mechanisms by which this occurs. The Specific Aims of this project are to: 1) determine the expression pattern of the membrane-bound mucins MUC1 and MUC4 in major and minor salivary glands and in different glandular secretions. 2) characterize the nature of the interactions between membrane-bound and gel-forming mucins and 3) investigate the role of MUC I and MUC4 in protection of oral epithelial surfaces from microbial attack.

描述（由申请人提供）：粘蛋白是大量的糖基化蛋白质，由胃肠道，呼吸和泌尿生殖区的上皮细胞合成和分泌。迄今为止，已经确定了13个不同的粘蛋白基因，可以将它们分为两种不同的类别，即凝胶形成的粘蛋白和膜结合的粘蛋白。在唾液分泌物中，粘蛋白促进言语和吞咽，保护口腔表面免受微生物，化学和机械损伤，并有助于有选择性的渗透性扩散屏障和外部环境之间。这些功能在很大程度上归因于唾液中的主要凝胶粘蛋白Mg1。然而，我们最近表明，在所有主要的唾液腺，包括腮腺，两个膜结合的粘蛋白MUC1和MUC4都表达。尽管几乎没有关于唾液腺和唾液中膜结合的粘蛋白的表达和功能特性知之甚少，但我们的假设是这些分子在保护口腔上皮表面中构成了口服宿主防御系统不可或缺的一部分。该项目的总体目标是了解发生这种情况的机制。 该项目的具体目的是：1）确定主要和小唾液腺中膜结合的粘蛋白MUC1和MUC4的表达模式以及在不同的腺体分泌中。 2）表征膜结合和形成凝胶的粘蛋白之间相互作用的性质，3）研究MUC I和MUC4在保护口腔上皮表面免受微生物攻击中的作用。