Sample Collection Protocols

样本采集协议

• 批准号: 7299840
• 负责人: Barney Scott Graham
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7299840
• 关键词: Sample; Collection; Protocols

This project consists of two protocols to collect human blood samples for research studies and to validate research assays used in evaluation of clinical trials immunogenicity results. VRC PAN allows collection of oral swabs, urine samples and blood samples through normal phlebotomy, while VRC 200 allows collection of plasma and blood cell samples by apheresis. The VRC is using the samples for several types of research, including to study specific immune responses to HIV infection in order to characterize protective responses that can prevent new infection in uninfected individuals, as well as define those responses that lead to successful control of viral replication in persons already infected with HIV. Because of the lack of an adequate animal model it is necessary to use human peripheral blood cells. We wish to define the specific human immune responses to HIV infection using human peripheral blood mononuclear cells (PBMCs) as a model. These sample collection protocols also support VRC research on other pathogens and vaccines.

该项目包括两种方案，用于收集人类血液样本进行研究，并验证用于评估临床试验的研究测定法，免疫原性结果。 VRC PAN允许通过正常的静脉切开术收集口服拭子，尿液样品和血液样本，而VRC 200允许通过放置分析收集血浆和血细胞样品。 VRC正在使用样品进行几种研究，包括研究对HIV感染的特定免疫反应，以表征可以防止未感染个体的新感染的保护性反应，并定义那些导致已经感染HIV患者的病毒复制的反应。由于缺乏适当的动物模型，因此有必要使用人类外周血细胞。我们希望使用人外周血单核细胞（PBMC）将特定的人类免疫反应定义为对HIV感染的特定反应。这些样本收集方案还支持对其他病原体和疫苗的VRC研究。