Open Tubular Capillary Electrochromatography/Mass Spectroscopy

开管毛细管电色谱/质谱

基本信息

批准号：
7193346
负责人：
JOSEPH J PESEK
金额：
$ 22.23万
依托单位：
SAN JOSE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-01-19 至 2010-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7193346
关键词：
Alkenes Alkynes Area Australia Biocompatible Biological Biological Markers Blood capillaries Carbon Cell physiology Cells Chemicals Chemistry Clinical Collaborations Complex Coupling Detection Development Devices Diagnosis Diagnostic Disease Drug Industry Drug Kinetics Enkephalins Future Generic Drugs Glycoproteins Goals High Pressure Liquid Chromatography Laboratories Link Liquid substance Mass Spectrum Analysis Methods Modeling Modification Numbers Object Attachment Operative Surgical Procedures Optics Particulate Peptides Pharmaceutical Preparations Pharmacologic Substance Phase Physiological Principal Investigator Process Proteins Proteomics Protocols documentation Range Reaction Reproducibility Resolution Sampling Silicon Silicon Dioxide Surface Techniques Technology Testing Therapeutic Agents Tissues Treatment Protocols Tubular formation Universities absorption base capillary chemical property chlorambucil/etoposide/lomustine design desire drug development drug production improved innovation medical schools method development professor programs small molecule solute success synthetic peptide tool two-dimensional

项目摘要

DESCRIPTION (provided by applicant): The prime objective of the project involves understanding open tubular capillary electrochromatography (OTCEC) in a specialized format: etching the inner wall of a fused silica capillary followed by chemical modification through silanization/ hydrosilation technology. The method offers a number of advantages over other configurations (packed capillaries, monoliths and other open tubular formats) of capillary electrochromatography. For fused silica capillaries in electrophoretic separations, the etching process described in the proposal results in a new more biocompatible surface that can be further modified by the silanization/ hydrosilation method to provide the selectivity desired. This combination leads to unique separation capabilities not possible with bare fused capillaries or those modified by other processes. Coupling OTCEC to mass spectroscopy offers analytical capabilities for the most complex samples such as those found in proteomics as well as many other biological and pharmaceutical applications. The development of more powerful techniques for chemical analysis is a crucial aspect to understanding diseases and to test the effects of various treatment protocols. This project focuses on providing a means for the analysis of complex samples found in clinical, biological and pharmaceutical applications.

描述（由申请人提供）：该项目的主要目的涉及以专用格式了解开放的管状毛细血管电解色（OTCEC）：蚀刻融合二氧化硅毛细管的内壁，然后通过硅烷化/氢化技术进行化学修饰。该方法具有比毛细血管电镀法的其他配置（包装的毛细管，整体和其他开放管状格式）相比。对于电泳分离中的融合二氧化硅毛细血管，该提案中描述的蚀刻过程导致了一种新的更生物相容性的表面，可以通过硅烷化/氢化方法进一步修改，以提供所需的选择性。这种组合会导致裸露的毛细血管或其他过程修改的毛细血管不可能带来独特的分离能力。将OTCEC与质谱耦合为最复杂的样品（例如蛋白质组学以及许多其他生物学和药物应用中发现的样品）提供了分析能力。化学分析更强大的技术的开发是理解疾病并测试各种治疗方案的影响的关键方面。该项目着重于提供一种分析临床，生物和药物应用中发现的复杂样品的方法。