Cellular Visualization and Analysis

细胞可视化和分析

• 批准号: 7311421
• 负责人: Thomas E Finger
• 金额: $ 17.02万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7311421
• 关键词: bioimaging /biomedical imaging; biomedical facility; cell type; chemoreceptors; confocal scanning microscopy; digital imaging; epithelium; histochemistry /cytochemistry; histology; image enhancement; image processing; molecular /cellular imaging

A major focus of research in the RMTSC laboratories is the use of specific molecular probes to delineate cellular composition and function in chemosensory systems. The major function of this core is to facilitate research of the participating investigators which relies on anatomically oriented methods to probe the organization, development or function of chemosensory epithelia or neural centers primarily involved in processing chemosensory information. The Core functions are encompassed by 3 specific Aims: 1. Support & Development of advanced histoiogical techniques 2. Confocal and Electron Microscopy 3. Quantitative Analysis and Visualization of Complex Anatomical Data Sets. Although each of the RMTSC laboratories utilizes anatomical techniques as a part of their overall research program, not all laboratories have equal access or experience in using advanced methodologies such as dual-labeling, functional imaging and electron microscopy. This Core facilitates sharing of expertise among the laboratories and makes available to all, specialized facilities available only at one of the participating Universities. Not only will the Core serve the local RMTSC investigators, but it will also serve as a resource for other chemosensory researchers especially in regards to development of software for mapping chemosensory representation in the brain.

RMTSC 实验室的一个主要研究重点是使用特定的分子探针来描绘化学感应系统中的细胞组成和功能。该核心的主要功能是促进参与研究人员的研究，这些研究人员依靠解剖学导向的方法来探测主要参与处理化学感应信息的化学感应上皮或神经中心的组织、发育或功能。核心功能包含 3 个具体目标： 1. 支持和开发先进的组织学技术 2. 共聚焦和电子显微镜 3. 复杂解剖数据集的定量分析和可视化。尽管每个 RMTSC 实验室都利用解剖技术作为其整体研究计划的一部分，但并非所有实验室都拥有使用双标记、功能成像和电子显微镜等先进方法的同等机会或经验。该核心促进实验室之间的专业知识共享，并向所有参与大学提供仅在其中一所大学提供的专业设施。该核心不仅可以为当地 RMTSC 调查人员提供服务，而且还可以作为一种资源 对于其他化学感应研究人员，特别是在开发绘制大脑化学感应表征的软件方面。