Vision Research Infrastructure Development

愿景研究基础设施开发

• 批准号: 7057290
• 负责人: HENRY Jerrold KAPLAN
• 金额: $ 18.06万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7057290
• 关键词: bioimaging /biomedical imaging; biomedical facility; cell type; confocal scanning microscopy; digital imaging; flow cytometry; fluorescence microscopy; histology; imaging /visualization /scanning; immunochemistry; mixed tissue /cell culture; tissue /cell preparation; vision

DESCRIPTION (provided by applicant): Although the Department of Ophthalmology and Visual Sciences at the University of Louisville has a large cadre of NEI funded vision scientists, it has not previously had funding for core module services. The purpose of this application is to provide our scientists with additional shared support to enhance their individual research capabilities and enhance further collaboration. Additionally, it is oriented to enhance the research capability of the University of Louisville for conducting vision research by encouraging collaborative studies and attracting other scientists to vision research. These goals will be met by operating two research modules which will provide the following services: l. The Image Acquisition and Analysis Module will provide technical support and expertise in histological procedures such as conventional histological processing, processing frozen tissue samples/blocks and immunohistochemical staining, confocal microscopy, etc. Additionally it will provide assistance and expertise in the acquisition, manipulation and analysis of digital images captured using various imaging devices such as confocal, bright field and fluorescence microscopy. II. The Flow Cytometry Module will help investigators identify specific cell types in mixed cell populations. Such studies include, but are not limited to, estimation of subsets of T-cells, determination of mitotic state and studies of cell surface receptors and markers. These services and resources will enhance vision research at the University of Louisville by extending the capability of NEI funded scientists, promoting collaboration and attracting new scientist to vision research.

描述（由申请人提供）：尽管路易斯维尔大学眼科和视觉科学系拥有一大批由 NEI 资助的视觉科学家骨干，但该系之前没有为核心模块服务提供资金。 此应用程序的目的是为我们的科学家提供额外的共享支持，以增强他们的个人研究能力并加强进一步的合作。此外，它还旨在通过鼓励合作研究和吸引其他科学家参与视觉研究来增强路易斯维尔大学进行视觉研究的研究能力。这些目标将通过运营两个研究模块来实现，这两个模块将提供以下服务： 湖图像采集和分析模块将提供以下方面的技术支持和专业知识： 组织学程序，例如常规组织学处理、处理冷冻组织 样品/块和免疫组织化学染色、共聚焦显微镜等。此外，它还会 提供数字图像采集、操作和分析方面的帮助和专业知识 使用各种成像设备（例如共焦、明场和荧光显微镜）捕获。 二.流式细胞术模块将帮助研究人员识别混合细胞群中的特定细胞类型。此类研究包括但不限于 T 细胞亚群的估计、有丝分裂状态的确定以及细胞表面受体和标记物的研究。 这些服务和资源将通过扩展 NEI 资助的科学家的能力、促进合作并吸引新科学家参与视觉研究来加强路易斯维尔大学的视觉研究。