A Novel Gnawing Assay to Quantify Nociception in Models of Chronic Orofacial Pain

一种量化慢性口面部疼痛模型伤害感受的新型啃咬试验

• 批准号: 7322090
• 负责人: Brian L Schmidt
• 金额: $ 19.25万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7322090
• 关键词: Address; Affect; American; Animal Disease Models; Animal Model; Animals; Arthralgia; Behavioral; Behavioral Assay; Biological Assay; Chronic; Clinical Trials; Condition; Custom; Data; Devices; Disease; Evaluation; Foundations; Goals; Head and Neck Cancer; Human; Impairment; Inflammation; Intractable Pain; Invasive; Investigation; Lead; Legal patent; Mastication; Masticatory muscles; Measurement; Measures; Methods; Modeling; Molecular; Myalgia; Nociception; Oral; Orofacial Pain; Pain; Pain Measurement; Patients; Personal Satisfaction; Research Personnel; Rodent; Rodent Model; Secondary to; Symptoms; Temporomandibular Joint; Temporomandibular Joint Disorders; Testing; Time; Trigeminal System; Work; base; cancer pain; chronic pain; design; improved; indexing; novel; novel therapeutics; orofacial

DESCRIPTION (provided by applicant): Chronic orofacial pain affects 10% of Americans and there currently is no effective treatment. The most common and most severe forms of orofacial pain include head and neck cancer pain, TMJ pain and masticatory muscle pain. The primary functional problem for orofacial pain patients is pain with chewing. However, there is no animal behavioral assay that quantifies the impact of nociception on oral function and that parallels the symptoms of patients. We hypothesize that chronic orofacial pain produces quantifiable masticatory changes. Moreover, we hypothesize that reduced gnawing function can be used as a measure of nociception. To test these hypotheses we have designed, custom built and pilot tested a novel device that objectively measures gnawing function in rodents. In this project we will use our novel device to quantify significant impairment of gnawing function in animal models of head and neck cancer pain, TMJ pain and masticatory muscle pain. To confirm that the altered gnawing function in the three models is secondary to pain we will demonstrate reversal with agents used clinically to treat these forms of chronic pain. We anticipate that our work will contribute fundamentally to improved understanding of nociceptive mechanisms of the trigeminal system by providing a quantitative functional nociceptive assay that does not depend on subjective behavioral interpretation and does not require observational time by an investigator.

描述（由申请人提供）：慢性口面疼痛影响10％的美国人，目前没有有效的治疗方法。最常见和最严重的口面痛形式包括头颈癌，TMJ疼痛和咀嚼性肌肉疼痛。口面疼痛患者的主要功能问题是咀嚼时疼痛。但是，没有动物行为分析可以量化伤害性对口服功能的影响，并且与患者的症状相同。我们假设慢性口面痛会产生可量化的咀嚼性变化。此外，我们假设降低的Gnawing功能可以用作伤害感受。为了测试这些假设，我们设计，自定义和试点测试了一种新型设备，该设备可以客观地测量啮齿动物的gnaw功能。在这个项目中，我们将使用新型装置来量化头颈癌，TMJ疼痛和咀嚼性肌肉疼痛的动物模型中gnaw功能的重大损害。为了确认三种模型中的gnaw函数的改变是疼痛的继发，我们将通过临床上用于治疗这些形式的慢性疼痛的药物证明逆转。我们预计，我们的工作将从根本上有助于改善三叉神经系统的伤害性机制，通过提供定量的功能性伤害性测定，该测定不取决于主观的行为解释，并且不需要研究者的观察时间。