A Novel Gnawing Assay to Quantify Nociception in Models of Chronic Orofacial Pain

一种量化慢性口面部疼痛模型伤害感受的新型啃咬试验

• 批准号: 7322090
• 负责人: Brian L Schmidt
• 金额: $ 19.25万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7322090
• 关键词: Address; Affect; American; Animal Disease Models; Animal Model; Animals; Arthralgia; Behavioral; Behavioral Assay; Biological Assay; Chronic; Clinical Trials; Condition; Custom; Data; Devices; Disease; Evaluation; Foundations; Goals; Head and Neck Cancer; Human; Impairment; Inflammation; Intractable Pain; Invasive; Investigation; Lead; Legal patent; Mastication; Masticatory muscles; Measurement; Measures; Methods; Modeling; Molecular; Myalgia; Nociception; Oral; Orofacial Pain; Pain; Pain Measurement; Patients; Personal Satisfaction; Research Personnel; Rodent; Rodent Model; Secondary to; Symptoms; Temporomandibular Joint; Temporomandibular Joint Disorders; Testing; Time; Trigeminal System; Work; base; cancer pain; chronic pain; design; improved; indexing; novel; novel therapeutics; orofacial

DESCRIPTION (provided by applicant): Chronic orofacial pain affects 10% of Americans and there currently is no effective treatment. The most common and most severe forms of orofacial pain include head and neck cancer pain, TMJ pain and masticatory muscle pain. The primary functional problem for orofacial pain patients is pain with chewing. However, there is no animal behavioral assay that quantifies the impact of nociception on oral function and that parallels the symptoms of patients. We hypothesize that chronic orofacial pain produces quantifiable masticatory changes. Moreover, we hypothesize that reduced gnawing function can be used as a measure of nociception. To test these hypotheses we have designed, custom built and pilot tested a novel device that objectively measures gnawing function in rodents. In this project we will use our novel device to quantify significant impairment of gnawing function in animal models of head and neck cancer pain, TMJ pain and masticatory muscle pain. To confirm that the altered gnawing function in the three models is secondary to pain we will demonstrate reversal with agents used clinically to treat these forms of chronic pain. We anticipate that our work will contribute fundamentally to improved understanding of nociceptive mechanisms of the trigeminal system by providing a quantitative functional nociceptive assay that does not depend on subjective behavioral interpretation and does not require observational time by an investigator.

描述（由申请人提供）： 10% 的美国人患有慢性口面部疼痛，目前尚无有效的治疗方法。最常见和最严重的口面部疼痛包括头颈癌疼痛、颞下颌关节疼痛和咀嚼肌疼痛。口面部疼痛患者的主要功能问题是咀嚼疼痛。然而，没有动物行为测定可以量化伤害感受对口腔功能的影响并且与患者的症状相一致。我们假设慢性口面部疼痛会产生可量化的咀嚼变化。此外，我们假设啃咬功能的降低可以用作伤害感受的衡量标准。为了测试这些假设，我们设计、定制并试点测试了一种新颖的设备，可以客观地测量啮齿动物的啃咬功能。在这个项目中，我们将使用我们的新型设备来量化头颈癌疼痛、颞下颌关节疼痛和咀嚼肌疼痛动物模型中啃咬功能的显着损伤。为了证实这三种模型中的啃咬功能改变是继发于疼痛的，我们将证明临床上用于治疗这些形式的慢性疼痛的药物的逆转作用。我们预计我们的工作将从根本上促进对三叉神经系统伤害感受机制的理解，通过提供不依赖于主观行为解释且不需要研究者观察时间的定量功能伤害感受测定。