Inflammatory Markers in a 2-Year Soy Intervention among Premenopausal Women

绝经前妇女两年大豆干预中的炎症标志物

• 批准号: 7320589
• 负责人: GERTRAUD MASKARINEC
• 金额: $ 6.92万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7320589
• 关键词: Accounting; Adipose tissue; Affect; Animals; Anti-Inflammatory Agents; Anti-inflammatory; Antioxidants; Area; Authorization documentation; Biological Assay; Biological Markers; Blood specimen; Body mass index; Breast Cancer Prevention; Breast Cancer Risk Factor; C-reactive protein; Cancer Etiology; Cells; Chronic; Control Groups; Daily; Diet; Dietary Intervention; Dropout; Effectiveness of Interventions; Enzyme-Linked Immunosorbent Assay; Estrogens; Ethnic Origin; Event; Fishes; Food; Freezing; Future; Genistein; Goals; Gonadal Steroid Hormones; Hepatic; Hormones; Human; Immune response; Immune system; Inflammation; Inflammatory; Inflammatory Response; Intake; Interleukin-6; Interleukins; Intervention; Isoflavones; Left; Leptin; Life; Linear Models; Link; Long-Term Effects; Luteal Phase; Malignant Neoplasms; Mammographic Density; Measurement; Measures; Mediating; Meta-Analysis; Mus; Numbers; Nutrient; Nutritional; Nuts; Omega-3 Fatty Acids; Ovulation; Pathway interactions; Premenopause; Preventive; Production; Protective Agents; Protein C; Protein Tyrosine Kinase; Publishing; Questionnaires; Randomized; Reporting; Research; Risk; Role; Sampling; Serum; Signal Transduction; Source; Soy Bean Curd; Soy Foods; Soy Milk; Soybeans; Specimen; Structure; Time; Topoisomerase; Treatment Protocols; Tumor Necrosis Factor-alpha; Tumor Necrosis Factors; Vegetables; Woman; Writing; adiponectin; base; cancer risk; carcinogenesis; cytokine; density; design; experience; human TNF protein; innovation; interest; malignant breast neoplasm; men; novel; protective effect; soy

DESCRIPTION (provided by applicant): The proposed project makes uses of existing serum samples collected during a 2-year randomized soy trial. Epidemiologic evidence supports a role for soy foods and isoflavones in breast cancer prevention, but the possible mechanisms of action are not well understood. Based on results from animal studies and human interventions, we hypothesize that soy reduces chronic inflammation, a possible risk factor for breast cancer. The original study and other reports indicate that the preventive effects of soy on breast cancer, if they exist, are not mediated by circulating sex hormones. The growing interest in an inflammatory etiology for cancer, as well as findings that link nutritional factors to immune responses, support our hypothesis. The aim of this study is to examine the effects of two daily servings of soy on serum levels of inflammatory markers. In addition, we will explore differences in serum levels of inflammatory markers by ethnicity, body mass index, and lifetime soy intake. During the 2-year nutritional intervention, 220 premenopausal women were randomized and donated multiple blood samples during the luteal phase as determined by an ovulation kit. The intervention group consumed two daily servings of soy foods, predominantly tofu, soy milk, and soy nuts, while the control women maintained their regular diet. According to several measures, compliance with the study regimen was excellent. The number of dropouts did not differ by group: 17 (15.6%) intervention women and 14 (12.6%) controls left the study prematurely. The subjects gave written permission to use their specimens for future analyses. Frozen serum samples stored at -80¿C will be analyzed for six inflammatory markers, adiponectin, leptin, interleukin (IL)-6, C-reactive protein (CRP), tumor necrosis factor (TNF)-a, and IL-1b, using established ELISA assays. For each of the 205 subjects (100 intervention subjects and 105 controls) with serum samples, we will analyze four samples, one each collected at baseline, after 6, 12, and 24 months. The statistical analysis will apply general linear models to compare the levels of inflammatory markers by soy intake, while taking into account the repeated measurement design.

描述（由申请人提供）： 拟议的项目利用在为期两年的随机大豆试验中收集的现有血清样品。流行病学证据支持大豆食品和异黄酮在预防乳腺癌中的作用，但尚不清楚可能的作用机制。根据动物研究和人类干预的结果，我们假设大豆降低了慢性感染，这是乳腺癌的可能危险因素。最初的研究和其他报告表明，大豆对乳腺癌的预防作用（如果存在）不是通过循环性马介导的。对癌症的炎症病因的兴趣日益增长，以及将营养因素与免疫反应联系起来的发现，支持我们的假设。这项研究的目的是检查大豆每天两份对血清炎症标志物水平的影响。此外，我们将通过种族，体重指数和终生大豆摄入量来探索炎症标记的血清水平差异。在两年的营养干预期间，由排卵试剂盒确定的在黄体期间随机分配了220名年绝经前妇女并捐赠了多个血液样本。干预组每天食用两份大豆食品，主要是豆腐，豆浆和大豆坚果，而对照妇女则保持常规饮食。根据几项措施，遵守研究方案非常好。组的辍学次数没有差异：17（15.6％）的干预妇女和14（12.6％）对照组过早地离开了研究。受试者给予书面许可，将其标本用于将来的分析。使用既定的ELISA分析，将对六个炎症标记，脂肪蛋白，瘦素，白介素（IL）-6，C反应蛋白（CRP），肿瘤坏死因子（TNF）-A和IL-1B进行六种炎症标记，脂肪蛋白，瘦素，白介素（IL）-6，CR蛋白，白介素（IL）-6和IL-1B，使用固定的ELISA分析，使用六个炎症标记物进行分析。对于具有血清样品的205名受试者（100名干预受试者和105个对照）中的每个受试者，我们将分析四个样本，每个样本在基线时在6、12和24个月后分析。统计分析将应用一般线性模型来比较大豆摄入量的炎症标记水平，同时考虑到重复的测量设计。