Engineering a motile biohybrid microrobot

设计可移动的生物混合微型机器人

• 批准号: 2898856
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2898856
• 关键词: Engineering; motile; biohybrid; microrobot

Liposomes are bioinspired particles which have tremendous potential fortargeted delivery of therapeutics. Motile liposomes that can actively propel themselves towards adiseased site are difficult to design. Some bacteria are known to actively migrate towards, target,actively penetrate and colonise tumours.Our proposition is that instead of designing motile liposomes from scratch, they can insteadhitchhike on motile bacteria to ferry them to discrete locations, allowing liposomes to reachotherwise inaccessible targets. In addition to delivery, the engineered bacteria will also form partof the therapy itself leading to degradation of the tumour extracellular matrix, allowing theliposomes to reach solid tumours.We will engineer the liposomes for controlled response to external stimuli based on membraneengineering (Elani) and engineer the liposomes with synthetic constructs enabling the liposomesto also act as a kill switch to ensure bacteria destruction after therapeutic delivery (Ceroni andPolizzi). We will also have a non-genetic external kill switch module to control safety, achieved byexploiting a suite of stimuli responsive liposomes have been developed over the past three years(light, heat, ultrasound, pH).

脂质体是仿生颗粒，在靶向治疗药物递送方面具有巨大潜力。能够主动将自身推向患病部位的活动脂质体很难设计。已知一些细菌会主动迁移、瞄准、主动渗透和定植肿瘤。我们的主张是，与其从头开始设计活动脂质体，不如它们搭上活动细菌的便车，将它们运送到离散的位置，从而使脂质体能够到达其他方式无法到达的目标。除了递送之外，工程细菌还将成为治疗本身的一部分，导致肿瘤细胞外基质降解，从而使脂质体到达实体肿瘤。我们将基于膜工程（Elani）和工程设计脂质体，以控制对外部刺激的反应具有合成结构的脂质体使脂质体还可以充当杀死开关，以确保治疗递送后细菌被破坏（Ceroni 和 Polizzi）。我们还将拥有一个非遗传的外部杀死开关模块来控制安全，这是通过利用过去三年开发的一套刺激响应脂质体（光、热、超声波、pH）来实现的。