The Respiratory Eff. Study of Aerosolized Red Tide Toxins in Humans

呼吸效应。

• 批准号: 7131156
• 负责人: LORA Elderkin FLEMING
• 金额: $ 35.38万
• 依托单位: UNIVERSITY OF NORTH CAROLINA WILMINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-08 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7131156
• 关键词: adolescence (12-20); adult human (21+); aerosols; asthma; biomarker; brevetoxin; chronic obstructive pulmonary disease; environmental exposure; environmental health; environmental toxicology; epidemiology; geographic difference; human subject; inflammation; longitudinal human study; patient oriented research; respiratory disorder epidemiology; respiratory function

Over the past 3 years, the Research Project 4 (RP4) Investigators have assembled a cohort of asthmatics and studied their respiratory responses to Florida red tide by comparing exposure and nonexposure periods. We have demonstrated statistically significant changes in inflammatory markers, respiratory function and reported symptoms in asthmatics with only 1 hour of acute beach exposure; no significant changes were observed during similar non-exposure periods. Parallel to these studies, the Investigators have assisted the Centers for Disease Control and Prevention (CDC) and the Florida Dept of Health (FDOH) in assembling and evaluating an occupational cohort. This represents the first time that possible health effects from exposure to aerosolized Florida red tide toxins have been evaluated epidemiologically in human populations. Over the next 5 years, working with the Toxin Probes and Assays Core, the Field Logistics and Development Core, and Project by Cheng, we propose to continue studying the exposures and health effects for humans due to aerosol exposures to Florida Red Tide, using new measures of exposure and physiologic effects. Specifically, we will annually collect pre- and post-exposure health effects and environmental exposure data at a time when a Florida red tide occurs and at a time with no Florida red tide for 1 hour of beach exposure. In addition, the investigators will annually conduct a 5 day Follow-up Study directly following the 1 hour Acute Exposure Study to collect data on persistent and delayed health effects from the acute exposure, as well as from possible repetitive exposures to Florida red tides toxins over 5 days. Additional data will be collected every 6 months to evaluate the severity and controllability of the asthmatics in the prior 6 months. The data from these annual studies (exposed and unexposed) will be evaluated longitudinally to explore possible chronic effects of varying Florida red tide toxin levels on the health of asthmatics, as well as subpopulations among the asthmatics. Based on the results of the animals models ( Projects by Benson and Abraham) and our epidemiologic work, we will use the following measurable physiologic effects: reported symptoms and measures of asthma controllability, spirometry, nasal swabs for inflammatory mediators, and exhaled condensates for acidification and selected cytokines, as well as the exposure measures of environmental and personal monitoring and nasal swabs for brevetoxins. The possible association between the uncontrollability of asthma associated with Florida red tide toxin exposure over time will be evaluated. In addition, the possible dose response relationship will be explored between long term exposure to Florida red tide toxins and their effects on measurable physiologic effects in asthmatics by evaluating coastal vs inland residents. Over the proposed 5 years, the project investigators will also take advantage of advancements in the science made by the other Projects to refine methods for measuring human exposures and physiologic effects from this environmental occurrence. The data from these studies will provide valuable medical and public health information concerning acute, subacute and chronic respiratory effects of aerosolized red tide toxin exposures annually in asthmatics over a 5 year period.

在过去 3 年里，研究项目 4 (RP4) 的研究人员聚集了一批 哮喘患者并通过比较暴露和未暴露的情况来研究他们对佛罗里达赤潮的呼吸反应 期间。我们已经证明炎症标志物发生了统计上显着的变化， 仅急性海滩暴露 1 小时的哮喘患者的呼吸功能和报告症状；不 在类似的非暴露期间观察到显着变化。与这些研究并行的是， 调查人员协助疾病控制与预防中心 (CDC) 和佛罗里达州部门 卫生部门 (FDOH) 收集和评估职业群体。这代表着第一次 已经评估了接触雾化佛罗里达赤潮毒素可能对健康造成的影响 在人群中的流行病学。 在接下来的 5 年里，通过与毒素探针和检测核心、现场后勤和开发核心以及 Cheng 的项目合作，我们建议 继续研究佛罗里达州气溶胶暴露对人类的暴露和健康影响 赤潮，使用新的暴露和生理效应测量方法。具体来说，我们每年都会收集 佛罗里达赤潮发生时暴露前和暴露后的健康影响以及环境暴露数据 发生时，海滩暴露时间为 1 小时，佛罗里达州没有出现赤潮。此外，调查人员 将每年在 1 小时的急性暴露研究之后直接进行 5 天的跟踪研究 收集有关急性暴露以及可能的持续和延迟健康影响的数据 5天内反复接触佛罗里达赤潮毒素。每 6 次就会收集一次额外数据 评估过去 6 个月内哮喘的严重程度和可控性。数据来自 这些年度研究（暴露的和未暴露的）将进行纵向评估，以探索可能的 不同佛罗里达赤潮毒素水平对哮喘患者健康的慢性影响，以及 哮喘患者的亚群。 根据动物模型的结果（本森和亚伯拉罕的项目）和我们的流行病学工作，我们 将使用以下可测量的生理效应： 报告的哮喘症状和测量 可控性、肺活量测定、鼻拭子检测炎症介质以及呼出冷凝物检测 酸化和选定的细胞因子，以及环境和个人的暴露措施 短尾毒素监测和鼻拭子。不可控性之间可能存在关联 将评估与佛罗里达赤潮毒素暴露一段时间相关的哮喘。此外， 将探讨长期暴露于佛罗里达赤潮之间可能的剂量反应关系 通过评估沿海与内陆毒素及其对哮喘患者可测量生理效应的影响 居民。在拟议的 5 年内，项目研究人员还将利用以下方面的进步： 其他项目所取得的科学成果，旨在完善测量人类暴露的方法和 这种环境事件的生理影响。这些研究的数据将提供 有关急性、亚急性和慢性呼吸道疾病的宝贵医疗和公共卫生信息 五年内每年接触气溶胶赤潮毒素对哮喘患者的影响。