Instrumentation Development on Multi-Scaled Scattering for Bio-Molecular Solution

生物分子解决方案多尺度散射仪器的开发

• 批准号: 7282455
• 负责人: Benjamin Chu
• 金额: $ 15.24万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7282455
• 关键词: Alzheimer' s Disease; Amyloid; Behavior; Biological; Cells; Collagen; Complex; Dependence; Development; Education; Electromagnetic Energy; Evolution; Fertilization; Funding; Gel; In Vitro; KLK3 gene; Knowledge; Laboratories; Lasers; Length; Light; Liquid substance; Measurement; Methods; Microscopy; Molecular; Molecular Conformation; Morphology; Motion; Muscle; PH-30 fusion protein; Peptides; Phase Transition; Photons; Production; Range; Reaction; Research; Resolution; Roentgen Rays; Sampling; Solutions; Source; Structure; Suspension substance; Suspensions; Synchrotrons; System; Systems Integration; Techniques; Testing; Time; Today; United States National Institutes of Health; Variant; Visible Radiation; X ray diffraction analysis; X-Ray Diffraction; base; beamline; chemical reaction; cyritestin; design; instrument; instrumentation; light intensity; light scattering; macromolecule; mineralization; research study; size

DESCRIPTION (provided by applicant): The present proposal is aimed at incorporating the newly developed cross-correlation light scattering capability with synchrotron X-ray scattering, specifically designed for bio-macromolecular gels, solutions and suspensions. The combined X-rays and laser light scattering techniques will be able to detect structural variations and dynamics in a continuous and very broad spatial range (0.1 - 2000 nm), even in turbid media. Many bio-macromolecular fluids, when undergoing structure changes or chemical reactions as well as phase transitions, can become opaque. Then, multiple scattering becomes a serious problem, especially in the visible light wave length range. The cross-correlation technique can extract single-scattering information in relatively turbid media, with the dynamic cross-correlation function analysis yielding knowledge on diffusive and rotational motions of species and estimates of size distributions. Such information, when simultaneously combined with X-ray scattering and diffraction results, is not attainable by methods such as NMR or microscopy. The unique multiple-scaled scattering instrumentation for research and education of complex bio- macromolecular solutions will include the integration of wide-angle X-ray diffraction (WAXD, spatial resolution 0.1-2 nm), small-angle X-ray scattering (SAXS, spatial resolution 2-120 nm) and laser light scattering with cross-correlation (spatial resolution 5-2000 nm). Experiments will include in-vitro bio- mineralization in collagen, muscle extension, solution behavior of bio-macromolecular aggregations, e.g., amyloids - the Abeta42 peptide involved in Alzheimer's disease; peptides derived from fertilinD and cyritestin in fertilization. The proposed light-scattering instrumentation, including a "constant-concentration" flow cell (for bio-mineralization study) and a small-volume solution cell (for precious bio-materials), will be assembled and initially tested at Stony Brook, and then incorporated into the X27C beam line in the National Synchrotron Light Source (NSLS), Brookhaven National Laboratory (BNL). The proposed instrumentation will be the first of its kind for combined laser light with cross-correlation and X-ray scattering research in the world. This instrumentation will provide new information, not accessible today, to several NIH projects described in this proposal; it will also be useful to many other NIH funded projects through general users of the X27C beamline.

描述（由申请人提供）：目前的建议旨在将新开发的互相关光散射能力与同步加速器X射线散射结合在一起，该散射专为生物分子凝胶，溶液和悬浮液而设计。联合X射线和激光光散射技术将能够在连续且非常宽的空间范围（0.1-2000 nm）中检测结构变化和动力学，即使在浑浊的介质中也是如此。许多生物大分子流体在发生结构变化或化学反应以及相变时，可能会变成不透明。然后，多个散射成为一个严重的问题，尤其是在可见的光波长度范围内。互相关技术可以在相对浑浊的介质中提取单散射信息，并具有动态互相关函数分析，从而使物种的扩散和旋转运动以及大小分布的估计值获得知识。当与NMR或显微镜之类的方法无法实现此类信息，同时与X射线散射和衍射结果结合在一起时。用于研究和教育复杂生物大分子溶液的独特多尺度散射仪器将包括整合广角X射线衍射（WAXD，空间分辨率0.1-2 nm），小角度X射线散射（SAX，SAX，，，，，，，，，X射线分辨率（SAX，SAX，，，，，）空间分辨率2-120 nm）和激光光散射具有互相关（空间分辨率5-2000 nm）。实验将包括胶原蛋白中的体外生物矿化，肌肉延伸，生物大分子聚集的溶液行为，例如淀粉样蛋白 - 涉及阿尔茨海默氏病的Abeta42肽；源自受精的肥料和甲状腺素的肽。所提出的光散射仪器，包括“恒定浓度”流动池（用于生物矿化研究）和小体积溶液细胞（用于珍贵的生物材料），并将在Stony Brook组装，然后在Stony Brook进行测试，然后进行测试。在布鲁克黑文国家实验室（BNL）的国家同步加速器光源（NSLS）中纳入X27C光束线。拟议的仪器将是世界上首次将激光光与互相关和X射线散射研究结合在一起。该仪器将向本提案中描述的几个NIH项目提供新的信息，而今天无法访问；它对许多其他NIH资助的项目也将通过X27C梁线的普通用户有用。