Media for Capillary and Micro-Chip Based Electrophoresis

用于毛细管和微芯片电泳的介质

基本信息

批准号：
6662015
负责人：
Benjamin Chu
金额：
$ 23.73万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
1995
资助国家：
美国
起止时间：
1995-09-30 至 2006-02-28
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The specific aim of the renewal proposal deals with the development and the optimization of better separation media for DNA capillary/microchip (array) electrophoresis. A conceptual model based on the DNA chain dynamics and fundamental principles of polymer physics, together with the aid of polymer synthesis, has been developed and is being tested. We propose to complete the tests, to design new separation media based on the model, and to put our separation media into practice. The essence of the conceptual model on polymer separation medium has been developed and tested out extremely well. Based on this model, the separation medium should be neutral, hydrophilic, and stable. It should also have surface-active properties. More importantly, in order to achieve a faster run time, the polymer chains should be more extended so that the same obstacles can be created with lesser amounts of polymers, implying that a lesser amount of polymers will be needed to create the same effective mesh size. Furthermore, in order to separate larger size fragments (e.g., DNAs; though not limited to DNAs alone), the polymer chain entanglement times should be as long as possible (so as to simulate a chemically cross-linked gel). What we have done and shall do is to demonstrate definitively the concepts of this model by using a set of test experiments since there are synthetic limitations to create an ideal separation medium. Furthermore, no quantitative theoretical treatment exists at this complex level, and in practice, most polymers do not mix well. Thus, with this new conceptual model, we hope to be able to provide the appropriate means to improve any existing and future separation media for capillary electrophoresis. Preliminary tests by Applied Biosystems on one of our more readily accessible separation media have already shown that it is better than the commercial separation medium currently being used by ABI.

描述（由申请人提供）：续展提案的具体目标致力于开发和优化更好的分离介质 DNA 毛细管/微芯片（阵列）电泳。基于的概念模型 DNA 链动力学和聚合物物理学的基本原理借助聚合物合成，已经开发出来并正在测试。我们建议完成测试，并在此基础上设计新的分离介质模型，并将我们的分离介质付诸实践。聚合物分离介质概念模型的本质是开发和测试得非常好。基于该模型，分离介质应是中性、亲水且稳定的。它还应该有表面活性特性。更重要的是为了实现更快的运行随着时间的推移，聚合物链应该更加延伸，这样同样的障碍就可以由较少量的聚合物制成，这意味着较少量的需要聚合物来创建相同的有效网格尺寸。此外，在为了分离较大尺寸的片段（例如 DNA；但不限于 DNA）单独），聚合物链缠结时间应尽可能长（因此以模拟化学交联凝胶）。我们已经做了什么和将要做什么是通过使用一组明确地证明该模型的概念测试实验，因为存在合成限制来创建理想的分离介质。此外，不存在定量理论处理这种复杂程度，在实践中，大多数聚合物都不能很好地混合。因此，随着对于这个新的概念模型，我们希望能够提供相应的手段改进任何现有和未来的毛细管分离介质电泳。应用生物系统公司对我们的更多产品之一进行了初步测试易于使用的分离介质已经表明它比 ABI 目前使用的商业分离介质。