CORE--BIOMATERIALS

核心--生物材料

• 批准号: 7073757
• 负责人: DARREN P. WALLACE
• 金额: $ 11.03万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7073757
• 关键词: animal care; biomaterials; biomedical facility; cell bank /registry; cell line; genetic registry /resource /referral center; genetically modified animals; genotype; human genetic material tag; human tissue; laboratory mouse; nucleic acid purification; polycystic kidney; protein purification; tissue /cell culture; tissue /cell preparation

The overall goal of the PKD Biomaterials Research Core is to establish and maintain a repository for human and animal biological materials for.investigators within the Kansas PKD Center. The core will provide resources and expertise for the development of high quality research materials, including primary cultures of epithelial cells, histological specimens, DNA, RNA and cell proteins from ADPKD, ARPKD and normal human kidney tissues. The core will also establish and maintain immortalized cell lines from mice with unique PKD gene mutations. The strength of the core is that it will provide a central laboratory with dedicated research staff experienced in 1) the procurement the human tissues and trained on the proper handling of the human biological samples, 2) methods of aseptic collection of cystic and non-cystic tissue and cyst fluid, 3) histological preparation 4) protein, RNA and DNA extraction, 5) animal husbandry, timed breeding and animal genotyping, and 6) preparation of cell lines from specific cell types for PKD mice. The specific aims of the Core are: Aim 1: Establish and maintain a human PKD biomaterial repository. Aim 2: Generate and maintain conditionally immortalized renal epithelial cell cultures from animals with unique gene defects that cause PKD. Aim 3: Provide technical support and training for in vitro methods for the investigation of the PKD cellular phenotype. The Core will provide unique PKD biomaterials to members of the Kansas PKD Center and to collaborators at the Kansas University Medical Center and other universities.

PKD 生物材料研究核心的总体目标是为堪萨斯 PKD 中心内的研究人员建立和维护人类和动物生物材料储存库。该核心将为开发高质量研究材料提供资源和专业知识，包括上皮细胞的原代培养物、组织学标本、来自 ADPKD、ARPKD 和正常人肾组织的 DNA、RNA 和细胞蛋白。该核心还将建立并维持来自具有独特 PKD 基因突变的小鼠的永生化细胞系。该核心的优势在于，它将提供一个中心实验室，配备在以下方面经验丰富的专门研究人员：1）采购人体组织并接受正确处理人体生物样本的培训，2）囊性和非囊性的无菌收集方法组织和囊肿液， 3）组织学制备4）蛋白质、RNA和DNA提取，5）畜牧业、定时育种和动物基因分型，以及6）针对PKD小鼠的特定细胞类型制备细胞系。核心的具体目标是： 目标 1：建立和维护人类 PKD 生物材料库。目标 2：从具有导致 PKD 的独特基因缺陷的动物中产生并维持条件永生化肾上皮细胞培养物。目标 3：为 PKD 细胞表型研究的体外方法提供技术支持和培训。该核心将为堪萨斯 PKD 中心的成员以及堪萨斯大学医学中心和其他大学的合作者提供独特的 PKD 生物材料。