Zeiss 510/META Confocal Microscope Plus Live Cell Chamber

Zeiss 510/META 共焦显微镜 Plus 活细胞室

• 批准号: 7216564
• 负责人: OLGA KOVBASNJUK
• 金额: $ 44.42万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7216564
• 关键词: 11 year old; Arts; Basic Science; Carbon Dioxide; Cells; Computer software; Computers; Core Facility; Deterioration; Development; Digestive System Disorders; Doctor of Medicine; Doctor of Philosophy; Fee-for-Service Plans; Fluorescence Resonance Energy Transfer; Funding; Housing; Image; Laboratories; Lasers; Life; Location; Microscope; Minor; Modeling; Monoclonal Antibody R24; National Institute of Diabetes and Digestive and Kidney Diseases; Other Resources; Physiological; Request for Applications; Research; Research Personnel; Scanning; Scientist; Speed; System; Technology; Temperature; Time; United States National Institutes of Health; Week; base; cellular imaging; computerized data processing; fluorescence imaging; improved; instrument; prevent; research facility

DESCRIPTION (provided by applicant): This application requests a Zeiss 510/META laser scanning confocal microscope with environmental chamber to replace our 11 year old Zeiss 410 microscope, which is part of the Imaging Core (Core Director, O. Kovbasnjuk, Ph.D., who is PI of this application) of an NIH NIDDK Core Development Center (R24, DK064388, "Hopkins Basic Science Digestive Diseases Core Center", PI, M. Donowitz, M.D.). The Zeiss 510/META microscope will allow us to provide state-of-the-art fluorescence imaging capabilities for 5 major interacting investigators, who are supported by the NIH NIDDK Core Development Center and who are closely interacting. There are also 6 minor users supported by the same Core Center who have their laboratories in the Ross Research Building, which houses the GI Core Center and the Zeiss confocal. In addition to being part of an NIH funded Core Center, the 510/META will be a resource for other JHUSOM scientists needing an environmental chamber on a confocal and will be available on a fee-for-service basis to scientists in a major Hopkins research facility (Ross Building). The 410 no longer satisfies our users' needs which can be met by the 510/META. This is due to 1) Deterioration of the Zeiss 410 PMT's and pin holes plus its outmoded software and tenuous computer support so the entire system will not be functional once the current computer fails. 2) Lack of spectral scanning capability needed for FRET applications. 3) Need for extensive use of FRET and FRAP with improved precision on 510 due to more precise pixel alignment than 410. The features of the Zeiss 510/META which are necessary to accomplish the diverse biomedical projects and which do not exist on the Zeiss 410 model to be replaced include: multitracking technology for speed and precise pixel localization; Zeiss LSM510 and Axiovision software for rapid signal processing and analysis; and a live cell chamber with temperature and CO2 controls needed for prolonged live cell imaging. The JHUSOM Imaging Core is not able to provide adequate time for the physiologic studies proposed in the R24 Center. This is preventing the proposed FRET studies and overnight constant temperature chamber studies. Importantly, no instruments available on a fee-for-service basis at JHUSOM are equipped with a live cell chamber. The Zeiss 510/META will be installed in the NIH NIDDK Core Center Imaging Core Facility, a location that is central to most of the users. It is anticipated that 75% of the 40 hr week will be used by the 5 major plus 6 minor users, with the remaining 25%, representing ~10-15 additional hrs/week, will be taken up by fee-for-service users, mostly from the Ross Building plus other Hopkins users needing the live cell chamber.

描述（由申请人提供）：本申请要求使用带有环境室的 Zeiss 510/META 激光扫描共焦显微镜来替换我们已有 11 年历史的 Zeiss 410 显微镜，该显微镜是成像核心的一部分（核心总监，O. Kovbasnjuk 博士） .（本申请的 PI））NIH NIDDK 核心开发中心（R24，DK064388，“霍普金斯基础科学消化疾病核心中心”，PI，M. Donowitz，医学博士）。 Zeiss 510/META 显微镜将使我们能够为 5 位主要的互动研究人员提供最先进的荧光成像功能，这些研究人员得到 NIH NIDDK 核心开发中心的支持，并且密切互动。还有 6 个由同一核心中心支持的未成年用户，他们的实验室位于罗斯研究大楼，该大楼设有 GI 核心中心和蔡司共焦。除了作为 NIH 资助的核心中心的一部分之外，510/META 还将成为其他需要共焦环境室的 JHUSOM 科学家的资源，并将以按服务收费的方式提供给霍普金斯大学一项主要研究的科学家设施（罗斯大厦）。 410 不再满足我们用户的需求，而 510/META 可以满足用户的需求。这是由于 1) Zeiss 410 PMT 和针孔的退化，加上其过时的软件和脆弱的计算机支持，因此一旦当前计算机出现故障，整个系统将无法正常工作。 2) 缺乏 FRET 应用所需的光谱扫描能力。 3) 由于像素对齐比 410 更精确，因此需要广泛使用 FRET 和 FRAP，并在 510 上提高精度。Zeiss 510/META 的功能是完成各种生物医学项目所必需的，而 Zeiss 410 上不存在这些功能被取代的模型包括：用于速度和精确像素定位的多跟踪技术； Zeiss LSM510和Axiovision软件用于快速信号处理和分析；以及具有长时间活细胞成像所需的温度和二氧化碳控制的活细胞室。 JHUSOM 成像核心无法为 R24 中心提出的生理研究提供足够的时间。这阻碍了拟议的 FRET 研究和过夜恒温室研究。重要的是，JHUSOM 提供的收费服务仪器均不配备活细胞室。 Zeiss 510/META 将安装在 NIH NIDDK 核心中心成像核心设施中，该设施是大多数用户的中心位置。预计每周 40 小时的 75% 将由 5 个主要用户和 6 个次要用户使用，其余 25%（即每周约 10-15 个小时的额外时间）将由按服务收费的用户占用，大部分来自罗斯大楼以及其他需要活细胞室的霍普金斯用户。