Purchase of a Linear Ion Trap/Fourier Transform Mass Spectrometer

购买线性离子阱/傅里叶变换质谱仪

基本信息

批准号：
7125654
负责人：
ROBERT James COTTER
金额：
$ 92.84万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2008-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7125654
关键词：
Acetylation Bioinformatics Brain Hypoxia-Ischemia Carbohydrates Charge Collaborations Communities Complex Mixtures Core Facility Data Dissociation Electrons Electrospray Ionization Fourier Transform Funding Gene Expression Glycopeptides Histones Housing Hybrids Individual Ions Laboratories Link Lysine Maps Mass Spectrum Analysis Methylation Modeling Modification N.I.H. Research Support Numbers Operative Surgical Procedures Participant Pathway interactions Peptide Fragments Peptides Phosphorylation Post-Translational Protein Processing Program Research Project Grants Protein Databases Protein Microchips Proteins Proteomics Request for Proposals Research Research Personnel Resolution Resources Role Services Site Source Structure Technology Training Transferase Two-Dimensional Gel Electrophoresis United States National Institutes of Health Work experience instrument mass spectrometer member peptide structure research facility

项目摘要

DESCRIPTION (provided by applicant): This proposal requests funds for a Thermoelectron (Sunnyvale, CA) model LTQ FT22000 hybrid linear ion trap/Fourier transform mass spectrometer, equipped with an electrospray ionization (ESI) source, infrared multiphoton dissociation (IRMPD) and electron capture dissociation (ECD). The instrument would directly benefit a group of major users all of whom have NIH research support and all of whom currently use mass spectrometry in their research. Many of the investigators are participants in one or more NIH-supported roadmap and program project grants, which incorporate mass spectrometric analysis as an integral part of the research effort and provide a means for expanding the number of investigators benefiting from these resources. This includes the Johns Hopkins Center for Proteomics of Ischemia and Hypoxia (N01 HV28180, supported by NHLBI) and the Johns Hopkins Technology Center for Networks and Pathways of Lysine Modifications (TCNP U54 RR020839, supported by NIH). All of the features and capabilities of this instrument, which distinguish it from those available on current instruments at Johns Hopkins, will be utilized. In work by several investigators on lysine modifications and the structures of histones, high mass resolution (50,000 to 100,000) will distinguish trimethyl and acetylation sites (which may exist at the same site on different molecules). ECD and IRMPD will be used to fragment the very large branched peptide structures resulting from SUMOylation, as well as very large, partially digested peptide fragments needed to map polyacetylated lysines in histones and histone transferases. ECD will also be critical for the structural analysis of carbohydrates, O-linked glycopeptides and negatively charged post- translational modifications, primarily phosphorylation. Many of the studies here are proteomics studies, utilizing protein databases for identification of proteins in complex mixtures (as well as their PTMs) from their digested peptides. High mass accuracy (1-5 ppm) is key to providing correct identifications. The instrument will be housed in and managed by the Middle Atlantic Mass Spectrometry Laboratory and operated by four individuals representing the Major Users and collaborators.

描述（由申请人提供）：该提案要求为热电子（加利福尼亚州桑尼维尔）型号 LTQ FT22000 混合线性离子阱/傅里叶变换质谱仪提供资金，该质谱仪配备电喷雾电离 (ESI) 源、红外多光子解离 (IRMPD) 和电子捕获解离 (ECD)。该仪器将直接惠及一群主要用户，他们都获得 NIH 研究支持，并且目前都在研究中使用质谱分析。许多研究人员都是 NIH 支持的一个或多个路线图和计划项目资助的参与者，这些资助将质谱分析作为研究工作的一个组成部分，并提供了一种扩大受益于这些资源的研究人员数量的方法。这包括约翰霍普金斯缺血和缺氧蛋白质组学中心（N01 HV28180，由 NHLBI 支持）和约翰霍普金斯赖氨酸修饰网络和途径技术中心（TCNP U54 RR020839，由 NIH 支持）。将利用该仪器与约翰·霍普金斯大学现有仪器不同的所有特性和功能。在几位研究人员对赖氨酸修饰和组蛋白结构的研究中，高质量分辨率（50,000 至 100,000）将区分三甲基和乙酰化位点（可能存在于不同分子的同一位点）。 ECD 和 IRMPD 将用于对 SUMO 化产生的非常大的分支肽结构进行片段化，以及绘制组蛋白和组蛋白转移酶中的多乙酰化赖氨酸图谱所需的非常大的部分消化的肽片段。 ECD 对于碳水化合物、O-连接糖肽和带负电的翻译后修饰（主要是磷酸化）的结构分析也至关重要。这里的许多研究都是蛋白质组学研究，利用蛋白质数据库从消化的肽中识别复杂混合物中的蛋白质（及其 PTM）。高质量准确度 (1-5 ppm) 是提供正确鉴定的关键。该仪器将安置在中大西洋质谱实验室并由其管理，并由代表主要用户和合作者的四名人员操作。