TANDEM TIME-OF-FLIGHT FOR PROTEOMICS ANALYSIS

用于蛋白质组学分析的串联飞行时间

基本信息

批准号：
6848330
负责人：
ROBERT James COTTER
金额：
$ 26.4万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-02-01 至 2006-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6848330
关键词：
analytical method antigens bioengineering /biomedical engineering biomedical equipment development biophysics chemical structure function glycolipids high throughput technology immune complex lipopolysaccharides mass spectrometry mathematics microarray technology miniature biomedical equipment proteomics

项目摘要

DESCRIPTION (provided by applicant): We propose to design, build and develop a compact tandem time-of-flight mass spectrometer for high throughput proteomics research based upon several technologies being developed in our laboratory. In particular the first mass analyzer (MSI) will utilize mass-correlated acceleration to focus ions with different kinetic energies and independently of mass to a focus point that is the entrance to the second mass analyzer (MS2). MS2 will be an endcap reflectron, which provides high order (nearly quadratic) kinetic energy focusing. The collision chamber will be located early on in the flight tube of MS1 as it is an essential feature of this instrument that there are no decelerating or re-accelerating fields, either constant or pulsed, between the ion source and the entrance to the endcap reflectron. Thus, fragment ions associated with a mass-selected precursor ion, and produced by collision-induced dissociation (CID) or by metastable decomposition, will be recorded at the same flight time. The high energy bandwidth endcap reflectron does not require stepping or scanning to record the entire product ion mass range in each time-of-flight cycle. In addition, because this reflectron also does not require reacceleration of after collision, it will be possible to utilize the full ion kinetic energy (10-20 keV) in collisional activation. The instrument will be utilized in a number of ongoing research projects involving peptidic and glycolipid antigens, the structures of lipid A, and protein arrays on a chip. In addition, a novel planar endcap reflectron design will be developed that is compatible with orthogonal acceleration. This will be used on an existing electron impact/orthogonal acceleration instrument, and will be further developed using atmospheric pressure MALDI as the ionization source. This technology is expected to bring high performance and tandem capabilities to a simple, compact instrument that can be utilized in proteomics research.

描述（由申请人提供）：我们建议设计、建造和开发一个用于高通量蛋白质组学的紧凑型串联飞行时间质谱仪基于我们实验室正在开发的多项技术的研究。在特别是第一个质量分析器（MSI）将利用质量相关加速聚焦具有不同动能的离子并且独立于质量到焦点，即第二质量分析器 (MS2) 的入口。 MS2 将是一个端盖反射器，提供高阶（接近二次）动能聚焦。碰撞室将位于早期 MS1 的飞行管，因为它是该仪器的基本特征没有减速或重新加速场，无论是恒定场还是脉冲场，在离子源和端盖反射器入口之间。因此，与质量选择的前体离子相关的碎片离子，并由碰撞诱导解离（CID）或通过亚稳态分解，将在同一飞行时间记录。高能带宽端盖反射器不需要步进或扫描来记录整个产物离子质量每个飞行时间周期的范围。另外，由于该反射器还碰撞后不需要重新加速，将有可能在碰撞激活中利用全部离子动能（10-20 keV）。该仪器将用于许多正在进行的研究项目涉及肽和糖脂抗原、脂质 A 的结构，以及芯片上的蛋白质阵列。此外，新颖的平面端盖反射器设计将开发与正交加速兼容的技术。这将是用于现有的电子撞击/正交加速仪器，以及将进一步开发使用大气压MALDI作为电离来源。该技术有望带来高性能和串联能力一种可用于蛋白质组学研究的简单、紧凑的仪器。