TANDEM TIME-OF-FLIGHT FOR PROTEOMICS ANALYSIS

用于蛋白质组学分析的串联飞行时间

• 批准号: 6620323
• 负责人: ROBERT James COTTER
• 金额: $ 24.8万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6620323
• 关键词: analytical method; antigens; bioengineering /biomedical engineering; biomedical equipment development; biophysics; chemical structure function; glycolipids; high throughput technology; immune complex; lipopolysaccharides; mass spectrometry; mathematics; microarray technology; miniature biomedical equipment; proteomics

DESCRIPTION (provided by applicant): We propose to design, build and develop a compact tandem time-of-flight mass spectrometer for high throughput proteomics research based upon several technologies being developed in our laboratory. In particular the first mass analyzer (MSI) will utilize mass-correlated acceleration to focus ions with different kinetic energies and independently of mass to a focus point that is the entrance to the second mass analyzer (MS2). MS2 will be an endcap reflectron, which provides high order (nearly quadratic) kinetic energy focusing. The collision chamber will be located early on in the flight tube of MS1 as it is an essential feature of this instrument that there are no decelerating or re-accelerating fields, either constant or pulsed, between the ion source and the entrance to the endcap reflectron. Thus, fragment ions associated with a mass-selected precursor ion, and produced by collision-induced dissociation (CID) or by metastable decomposition, will be recorded at the same flight time. The high energy bandwidth endcap reflectron does not require stepping or scanning to record the entire product ion mass range in each time-of-flight cycle. In addition, because this reflectron also does not require reacceleration of after collision, it will be possible to utilize the full ion kinetic energy (10-20 keV) in collisional activation. The instrument will be utilized in a number of ongoing research projects involving peptidic and glycolipid antigens, the structures of lipid A, and protein arrays on a chip. In addition, a novel planar endcap reflectron design will be developed that is compatible with orthogonal acceleration. This will be used on an existing electron impact/orthogonal acceleration instrument, and will be further developed using atmospheric pressure MALDI as the ionization source. This technology is expected to bring high performance and tandem capabilities to a simple, compact instrument that can be utilized in proteomics research.

描述（由申请人提供）：我们建议设计，建造和开发 高吞吐量蛋白质组学的紧凑型串联飞行时间质谱仪 基于我们实验室开发的几种技术的研究。在 特别是第一个质量分析仪（MSI）将利用质量相关 加速具有不同动能的聚焦离子，并且独立于 质量到焦点是第二质量分析仪（MS2）的入口。 MS2将是一个端盖Reflectron，它提供高阶（几乎二次） 动能聚焦。碰撞室将在 MS1的飞行管，因为它是该乐器的重要特征 没有减速或重新加速磁场，无论是恒定还是脉冲， 在离子源和端盖反射量的入口之间。因此， 与质量选择的前体离子相关的碎片离子，并由 碰撞引起的解离（CID）或通过亚稳态分解，将是 在同一飞行时间记录。高能带宽端盖反射负体 不需要步进或扫描来记录整个产品离子质量 每个飞行时间周期的范围。另外，因为此反射率也 碰撞后不需要重新计算 在碰撞激活中利用完整的离子动能（10-20 KEV）。 该仪器将用于许多正在进行的研究项目 涉及肽和糖脂抗原，脂质A的结构和 芯片上的蛋白质阵列。此外，一种新颖的平面端盖反射量设计 将开发与正交加速度兼容的。这将是 用于现有电子撞击/正交加速仪器，以及 将使用大气压力MALDI作为电离进一步开发 来源。 预计该技术将带来高性能和串联功能 可以在蛋白质组学研究中使用的简单，紧凑的仪器。